GBC sequence

Ashu Seq

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Oct 13, 2017, 9:47:26 AM10/13/17

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Dear All,

I was looking for the GBC barcode sequences used in the paper, could you please make them available?

Thank you,

Ashu

Ashu Seq

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Oct 25, 2017, 10:32:47 AM10/25/17

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Dear Moderators,

I have been looking for the GBC sequences used in the paper and I could not find them anywhere. I will be very happy if you indicate where I can get them. Alternatively, I will be very happy if you tell me the important points in designing them, the length etc.

Thank you again for all your cooperation,

With kind regards,

Ashu

Atray Dixit

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Oct 30, 2017, 6:46:44 PM10/30/17

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Hi Ashu,

Sorry for the delay in response. Attached are the GBC sequences for our PMDC screen. These GBCs were not designed specifically, rather they are a random sample of a random oligonucleotide pool.

In general, 15-20 bases will be sufficiently long that there will be an extremely low probability of observing GBC sequences that are within one or two mismatches of one another. If you are planning on designing GBCs it is also worth avoiding sequences containing restriction sites for subsequent sgRNA cloning (but this will not occur very often).

Hope that helps!

Atray

dc_pool.csv

Ashu Seq

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Nov 9, 2017, 4:08:07 AM11/9/17

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Thank you Atray!

That was helpful!

With kind regards,

Ashu

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