Centrifuge 5415d

[Ailene Goldhirsh]

TheEppendorf 5415Ddigital table top microfuge is extremely easy to use with knob control, virtually working as a turn key, walk away machine. Its small footprint andbrushless motor conveniently make it maintenance free. The 5415Dhas a maximum speed of 13,200 rpm and has a digital timer run time of up to 30minutes. The Eppendorf 5415D has a maximum capacity of up to 24 x 1.5ml tubes (or 36 x 0.5ml). With its space-saving design and user-friendly operating concept, the centrifuge is ideal for everyday routine tasks.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Please confirm this rotor meets your application needs and request a different rotor, if necessary. Available rotors can be found in our Rotor Compatibility Guide.

Centrifugation and Eppendorf: The stuff that legends are made of. And one such legend in the making is the new Centrifuge 5415 D, the logical successor to the classic 5415 C, the unrivalled model of longevity and ease of use in laboratories, clinics and research institutes throughout the world. The 5415 D has all the tried and tested technical features, including the almost indestructible maintenance-free drive. It goes without saying that the easy-to-follow digital display shows all significant parameter settings at a glance.

The centrifuge can be operated using the same 24-position standard rotor for 1.5 ml and 2 ml tubes or with the 36-position special rotor for 0.5 ml tubes. With or without refrigeration - the right choice is always 5415!

Product Information table border-collapse: collapse; width: 100%; th, td border: 1px solid #dddddd; padding: 8px; text-align: left; th background-color: #f2f2f2; The Eppendorf 5415D Centrifuge is a high-performance laboratory equipment designed for precise separation of particles from suspensions based on density. This German-made centrifuge features a maximum rotational speed of 14000 rpm, generating a kinetic energy of 3,100 Nm. It is capable of functioning under an ambient temperature range of 2C to 35C and a maximum relative humidity of 75%. This centrifuge was manufactured in 1996 and is in very good used condition, tested and proven to still function as intended despite some cosmetic damage. It is often used in molecular biology, biochemistry, and pharmaceutical laboratories to separate components of a complex mixture such as DNA, RNA, proteins, or cell organelles. It also finds applications in routine clinical and environmental sample processing.

The Centrifuge 5415 D is a non-refrigerated bench-top centrifuge and the Centrifuge 5415 R is a refrigerated bench-top centrifuge. Both are used in research and routine laboratories in the biosciences, industry, clinics and chemistry.

The Eppendorf 5415 series centrifuges have been discontinued by the manufacturer and replaced by the 5424 and 5424 R models. However, a number of units are always available for sale on LabX. Rotors and accessories for the 5415 D/R are still available for sale from Eppendorf, or there is a wide selection of rotors and parts available on LabX as well.

Find online auctions and classified ads for Eppendorf products and more on the LabX Marketplace. Browse ads and contact the seller directly or request a quote for more details. Buy and Sell Eppendorf Equipment and Accessories today on LabX!

For non-chiller units, remove the four side screws, and the entire housing should come right off. If not, turn it upside down and note the rusty crust that's gluing the metal frame to the plastic! Slide a knife, or pry to break the bond. Then invert again, lift the housing carefully and reach inside to remove the ribbon cable from the PCB (pinch the plastic connector sides to release.) There are also two ground cables and the release-string to remove. (Also note, if you want to remove the main PCB, must unscrew the four screws on the BOTTOM of the centrifuge, not the four screws on the PCB itself.

I've now seen more than one Eppendorf lab centrifuge with the same problem: sudden death, as if a fuse has blown, but the fuses are fine.The LCD remains blank. Simple cure: increase a 2.2uF capacitor value to 10uF or higher (but stay below 100uF.) This fixes an apparent problem of slow-aging of the "boot-circuit" in the main switching supply.

DETAILSIn both dead units the cause was the same: switching supply failure when the "bootstrap" startup circuit can't run the supply, but goes into constant resetting. If the supply could just start itself up once, everything else works fine. In the past with other systems, this failure comes from dead dried-out electrolytic capacitors. But replacing the large 2.2uF and 220uF caps in the centrifuge didn't help, and the values of the original components were OK.

Next I look at the circuitry around the UC2844 (which is live, lethal. Floating-at-170VDC-plus-120v). I use a (electrically floating, dangerous!) scope to monitor the 16-volt input to the Vcc pin of the UC2844 controller chip. (This Vcc is also found at the input pin of that other, non-isolated LM7805 regulator in the center of the board. The regulator tab is also the chip's common.) A 2nd scope channel monitors the voltage generated by the high-freq transformer, found at the cathode of a SMT diode next to the 220uF cap. During each restart, the 2.2uF cap must rise to 16V, then the chip turns on, and the little VOGT 50KHz transformer should take over, charging up the adjacent, larger 220uF cap, and power everything. On the scope, the 2.2uF does rise to 16V, turning on the UC2844. That voltage now decreases rapidly, since the 2.2uF is acting as the entire power supply. The 50KHz switcher runs for a moment, and it's output voltage (across the 220uF) rises rapidly. But before it has time to rise to 10V, the voltage on the 2.2uF has fallen below 10V, and the UC2844 shuts down. Then repeat the whole sequence. But, it just missed normal startup byabout half a volt! One cap voltage falls, the other rises, and they must pass each other in order to turn on the supply. There's not enough millijoules in the 2.2uF to last for the whole millisecs needed to boot up the switching supply.

So, maybe there's a shorted turn in that VOGT high-freq transformer block. Or maybe the LCD front panel is now drawing a few mA extra during the rising of the isolated 5V supply. Or maybe it was always supposed to be a 22uF capacitor, not 2.2uF? The voltages and RC periods are very close to the edge of failure, and any little change can push it over the threshold. Could I try replacing the little brown VOGT high-freq transformer? Nope, not available. So, kludge it: swap the 2.2uF capacitor with 10uF 25V tantalum. Yep, that did it. Starts up now just fine. (Hmm, in hindsight I probably should have used a 22uF or 47uF rather than a 10uF, just to keep the same failure from happening again in a couple of years!) ANOTHER TRICK: stand up the capacitor on 1cm leads, then to keep it cool, bend it away from the hot TO-220.

The Eppendorf 5415D digital table top microfuge is extremely easy to use with knob control, virtually working as a turn key, walk away machine. Its small footprint and brushless motor conveniently make it maintenance free. The 5415D has a maximum speed of 13,200 rpm and has a digital timer run time of up to 30 minutes. The Eppendorf 5415D has a maximum capacity of up to 24 x 1.5ml tubes (or 36 x 0.5ml). With its space-saving design and user-friendly operating concept, the centrifuge is ideal for everyday routine tasks.

Eppendorf 5415 is best known for its high quality construction, minimal foot prints, and high degree of flexibility. With its broad choice of high performance rotors, this low cost centrifuge can be used to perform in application areas such as routine clinical laboratory and biotechnological research.

The Eppendorf type 5145D centrifuge is a non-refrigerated bench-top centrifuge that is ideal for the simultaneous centrifugation of 24 Eppendorf micro test tubes at a fixed angle of 45 at a speed of up to 13200 rpm.

This centrifuge is also equipped with a timer, which makes timed centrifugation possible. In addition, the Eppendorf 5145D is used in research and routine laboratories in the life sciences, industry, clinics and chemistry.

Centrifuge 5415 R has the same user friendliness and all the tried and tested technical features of the non-refrigerated 5415 D, including the almost indestructible maintenance-free drive, which requires only 13 seconds to accelerate to the maximum speed of 13,200 rpm (max. rcf: 16,000 x g).

The rotor runs so smoothly that particles that have already been pelleted are not unintentionally resuspended. Centrifuge 5415 R enables rapid and reliable refrigerated centrifugation in a temperature range between 0 C and 40 C. Temperature-sensitive samples are maintained safely at 4 C, even at maximum speed. In the stand-by mode, the centrifuge then can be set to the desired temperature. With the aid of the Fast Cool function, refreshing 4 C can be attained within a mere 16 minutes. One undisputed innovation on Centrifuge 5415 R is the new motorized lid latch. Two built-in gripper arms close

the newly designed lid automatically.

Refrigerated tabletop centrifuges are quite expensive. Although discontinued, a refurbished Eppendorf 5415R (the model we use) still sells for $1-2K. The second of our two Eppendorf 5415R broke already more than a year ago and since then, we have been either centrifuging in the cold room or using the neighboring lab's cold fudge. But having two broken centrifuges of the same type, but different failure symptoms begged to assemble one functioning out of the remains.

One of the centrifuge rotors was stuck (could not be turned even by hand) and the other fuge was giving sparks and smoke when starting a run. I had postponed this repair since I thought it might take much time. However, when I opened the first centrifuge, I immediately could identify the part that I needed to graft from the other fuge. The whole repair lasted less than one hour. There was a blown resistor next to the cooling fan, which was easily replaced with the part from the second fuge. You can see the broken brass-colored resistor (CGS HSA50 resistor, available from Amazon for $13) in the front of the picture left to the rotor.

3a8082e126