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PeptideShaker
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PeptideShaker
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Welcome to the PeptideShaker user group!
PeptideShaker is a free tool dedicated to the processing of proteomics identifications.
You can find complete information and downloads at
http://compomics.github.io/
projects/peptide-shaker.html
.
For reporting specific issues or bugs, please use the issue trackers at
https://github.com/compomics/
peptide-shaker/issues
or
https://github.com/compomics/
searchgui/issues
.
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Swamy swaroop
,
Harald Barsnes
2
11/21/22
MALDI TOF data analysis
Hi, Sorry for the late answer! Seems like there was an error in the notification system for the
unread,
MALDI TOF data analysis
Hi, Sorry for the late answer! Seems like there was an error in the notification system for the
11/21/22
Wannes Dermauw
,
Harald Barsnes
2
11/21/22
"Default protein report"
Hi Wannes, Sorry for the very late answer! Seems like there was an error in the notification system
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"Default protein report"
Hi Wannes, Sorry for the very late answer! Seems like there was an error in the notification system
11/21/22
volkan yildirim
,
Harald Barsnes
2
11/21/22
MSConvert parameters for mzml files
Hi Volkan, Sorry for the very late answer! Seems like there was an error in the notification system
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MSConvert parameters for mzml files
Hi Volkan, Sorry for the very late answer! Seems like there was an error in the notification system
11/21/22
Mohammed Alhadidy
,
Harald Barsnes
2
11/21/22
Digestion with 2 enzymes
Hi Mohammed, Sorry for the very late answer! Seems like there was an error in the notification system
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Digestion with 2 enzymes
Hi Mohammed, Sorry for the very late answer! Seems like there was an error in the notification system
11/21/22
Ruben Bakker
,
Harald Barsnes
2
11/26/21
merging PSM reports
Hi Ruben, Splitting up the processing of the samples will make a (most likely) minor impact on the
unread,
merging PSM reports
Hi Ruben, Splitting up the processing of the samples will make a (most likely) minor impact on the
11/26/21
Ruben Bakker
,
Harald Barsnes
5
6/17/21
spectral mapping rate
Thank you for your explanations Harald! Best regards, Ruben On Wednesday, June 16, 2021 at 10:24:49
unread,
spectral mapping rate
Thank you for your explanations Harald! Best regards, Ruben On Wednesday, June 16, 2021 at 10:24:49
6/17/21
Deepti Jaiswal
,
Harald Barsnes
2
5/27/21
New peptide shaker version doesn't open the example
Hi, Thanks for letting us know. Please see https://github.com/compomics/peptide-shaker/issues/461 for
unread,
New peptide shaker version doesn't open the example
Hi, Thanks for letting us know. Please see https://github.com/compomics/peptide-shaker/issues/461 for
5/27/21
Zach Flaten
,
Harald Barsnes
2
4/27/21
Issues Loading PRIDE datasets via Reshake
Hi Zach, I'm afraid it seems like the web service PeptideShaker uses to access the PRIDE data is
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Issues Loading PRIDE datasets via Reshake
Hi Zach, I'm afraid it seems like the web service PeptideShaker uses to access the PRIDE data is
4/27/21
Harald Barsnes
1/27/21
SearchGUI update checker now working again
Hi all, We've just released a new version of SearchGUI (v4.0.16) that fixes a bug in the
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SearchGUI update checker now working again
Hi all, We've just released a new version of SearchGUI (v4.0.16) that fixes a bug in the
1/27/21
Aik Han Tan
,
Harald Barsnes
3
1/21/21
Error while exporting customised report
That works perfectly great! Thanks Harald. Best regards, Aik Han On Thu, Jan 21, 2021 at 7:32 PM
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Error while exporting customised report
That works perfectly great! Thanks Harald. Best regards, Aik Han On Thu, Jan 21, 2021 at 7:32 PM
1/21/21
Harald Barsnes
2
11/4/20
New releases: SearchGUI 4.0.0 and PeptideShaker 2.0.0
Hi again, I forgot to mention that the new releases require Java 9 or newer. So if you're still
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New releases: SearchGUI 4.0.0 and PeptideShaker 2.0.0
Hi again, I forgot to mention that the new releases require Java 9 or newer. So if you're still
11/4/20
tutagem tutagem
,
Peter H
2
8/26/20
IDA experiment and SWATH Acqusition
To search SWATH files you need to use a different pipeline. You can make your own library and search
unread,
IDA experiment and SWATH Acqusition
To search SWATH files you need to use a different pipeline. You can make your own library and search
8/26/20
Jiancheng Ding
, …
Harald Barsnes
5
6/17/20
Import MaxQuant result into Peptide-Shaker
Hi, If you have Thermo raw files you can use either ThermoRawFileParser (command line: https://github
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Import MaxQuant result into Peptide-Shaker
Hi, If you have Thermo raw files you can use either ThermoRawFileParser (command line: https://github
6/17/20
matte...@libero.it
,
Harald Barsnes
2
6/1/20
Load data into the program
Hi, If you have Thermo raw files you can convert them with ThermoRawFileParser, either via the
unread,
Load data into the program
Hi, If you have Thermo raw files you can convert them with ThermoRawFileParser, either via the
6/1/20
Vanessa Casas
,
Harald Barsnes
6
4/10/20
Memory issue
Hi Vanessa, Here is an updated beta version that should solve the export issues: http://vedlegg.uib.
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Memory issue
Hi Vanessa, Here is an updated beta version that should solve the export issues: http://vedlegg.uib.
4/10/20
Carlo de Oliveira Martins
,
Harald Barsnes
6
2/7/20
Confidence is not calculated - does not find the decoys
Thank you! Best regards On Fri, 7 Feb 2020 at 14:58, Harald Barsnes <harald....@gmail.com>
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Confidence is not calculated - does not find the decoys
Thank you! Best regards On Fri, 7 Feb 2020 at 14:58, Harald Barsnes <harald....@gmail.com>
2/7/20
Juan Camilo Rojas Echeverri
,
Harald Barsnes
5
2/4/20
MyriMatch error (Version: SearchGUI 3.3.17)
Hi Harald, Here I attach the parameters file I have been using. The PTMs were based on derivatization
unread,
MyriMatch error (Version: SearchGUI 3.3.17)
Hi Harald, Here I attach the parameters file I have been using. The PTMs were based on derivatization
2/4/20
Volkix
,
Harald Barsnes
2
1/24/20
Unknown aminoacids (X) in fasta file
Hi Volkan, Having X's in your sequence should not be an issue. Which version of SearchGUI are you
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Unknown aminoacids (X) in fasta file
Hi Volkan, Having X's in your sequence should not be an issue. Which version of SearchGUI are you
1/24/20
Juan Camilo Rojas Echeverri
, …
Harald Barsnes
5
1/16/20
PeptideShaker project generation error and Santa wish!
Hi Juan, > I'm guessing you have done a lot of new things with the beta version, but could you
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PeptideShaker project generation error and Santa wish!
Hi Juan, > I'm guessing you have done a lot of new things with the beta version, but could you
1/16/20
William Stone
,
Marc Vaudel
4
12/10/19
NSAF reported by ProteinShaker
Hi again, Thanks for your answer and the kind words on our work! If you want to normalize to the sum
unread,
NSAF reported by ProteinShaker
Hi again, Thanks for your answer and the kind words on our work! If you want to normalize to the sum
12/10/19
Harald Barsnes
,
René Zahedi
2
11/29/19
Announcement
PeptideShaker v2.0.0-beta and SearchGUI-4.0.0-beta now available for testing!
That's great news! Best. René On Fri., Nov. 22, 2019, 8:23 am Harald Barsnes, <harald.barsnes@
unread,
Announcement
PeptideShaker v2.0.0-beta and SearchGUI-4.0.0-beta now available for testing!
That's great news! Best. René On Fri., Nov. 22, 2019, 8:23 am Harald Barsnes, <harald.barsnes@
11/29/19
Kelvin Chung
, …
Harald Barsnes
5
11/8/19
The routine workflow for layman
Harald and JC Thank you very much for your reply. I understand the peptide shaker is a much more
unread,
The routine workflow for layman
Harald and JC Thank you very much for your reply. I understand the peptide shaker is a much more
11/8/19
HFaz
, …
Marc Vaudel
6
8/27/19
PeptideShaker for HPC cluster
Hi Innocent, After SearchGUI, PeptideShaker creates a unified set of peptide-spectrum matches. This
unread,
PeptideShaker for HPC cluster
Hi Innocent, After SearchGUI, PeptideShaker creates a unified set of peptide-spectrum matches. This
8/27/19
Juan Camilo Rojas Echeverri
,
Harald Barsnes
2
6/28/19
Separate files vs. merged files (.mgf) analysis
Hi JC, > Is there anything in the validation steps of PeptideShaker that you could see could cause
unread,
Separate files vs. merged files (.mgf) analysis
Hi JC, > Is there anything in the validation steps of PeptideShaker that you could see could cause
6/28/19
Juan Camilo Rojas Echeverri
,
Harald Barsnes
4
6/28/19
Export "Spectrum IDs" tab
Hi JC, > I tried before creating a customizable export that contained the intensity of the
unread,
Export "Spectrum IDs" tab
Hi JC, > I tried before creating a customizable export that contained the intensity of the
6/28/19
Bassel
,
lnnrt
3
4/2/19
FDR small data set size
Thanks a lot Innrt for your detailed answer. Very Helpful! Best, Bassel On Monday, March 25, 2019 at
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FDR small data set size
Thanks a lot Innrt for your detailed answer. Very Helpful! Best, Bassel On Monday, March 25, 2019 at
4/2/19
Dmitry Abashkin
,
Marc Vaudel
6
1/18/19
Peptide confidence calculation
Dear Dmitry, Thanks for the kind words. CoIP are tricky, I often see the bait alone or half of
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Peptide confidence calculation
Dear Dmitry, Thanks for the kind words. CoIP are tricky, I often see the bait alone or half of
1/18/19
Sameh Magdeldin
, …
Antonio Ortega
4
12/11/18
H/L ratio setting in dimethyl labeling is missing
Dear Sameh. I faced a similar problem, performing quantification on label-free DDA data, a few months
unread,
H/L ratio setting in dimethyl labeling is missing
Dear Sameh. I faced a similar problem, performing quantification on label-free DDA data, a few months
12/11/18
Stoyan Stoychev
,
Harald Barsnes
3
11/30/18
Db toolkit
Thanks Sent from my Sony Xperia™ smartphone ---- Harald Barsnes wrote ---- Hi Stoyan, You can find
unread,
Db toolkit
Thanks Sent from my Sony Xperia™ smartphone ---- Harald Barsnes wrote ---- Hi Stoyan, You can find
11/30/18
valentine....@gmail.com
,
Harald Barsnes
8
11/14/18
Fractions in peptide shaker
Hi Valentine, I don't see anything obviously wrong with your search parameters. And unless your
unread,
Fractions in peptide shaker
Hi Valentine, I don't see anything obviously wrong with your search parameters. And unless your
11/14/18