R-DNA

[Kimberley Swaney]

Hey would you mind helping me answer these questions. I answered them
on the worksheet, and I feel that I need to condense my answer and
make it more to the point.

I defined Recombinant DNA as: DNA that has been joined from two or
more different sources by molecular cloning.
(Is that all I need?)

Briefly describe the process that produces r-DNA?


The first step is to select a piece of DNA to be inserted
into a vector. The second step is to cut that piece of DNA with a
restriction
enzyme and then ligate the DNA insert into the vector with DNA Ligase.
Usually it is then inserted into bacteria (as they mutiple and divide
very quickly.)

(Is that all I need?)

As well is there anyway during Transciption or Translation that a
mutation can be indentified and corrected?

Thanks!

[Scott Harkness]

rDNA definition is great


All you need to know for the process is that DNA of one organism is
inserted into the genome (DNA) of another organism.



Errors in transcription and translation are not a big problem. They
do happen in the body but the result is a non-functional protein.
There are enough proteins that are made properly to still operate
normally. The big problem comes when there is an error/ mutation
during DNA replication. That means the template/ instructions to make
a specific is wrong and all of the mRNA made based on that gene will,
most likely, not work.

You will learn about what cells do if there are errors in the DNA in
university. Its called programmed cell death (apoptosis). The cell
destorys itself