hi there,
It is worth spending some time to learn some basic principles of molecular evolutionary analysis before trying to run PAML. I also recommend looking at some examples of how other people have successfully used PAML analysis for other genes. That will help you understand the types of questions PAML is able to answer.
It looks like your alignment contains the sequences of 8 totally different regions that are unalignable. We typically use PAML to analyze a single gene at one time, for example using several aligned orthologs from different species for a single gene. Or perhaps you might include paralogs of genes in a gene family, but you would not analyze unrelated genes together with each other. So maybe you want to collect orthologous sequences for each of your genes before you run PAML?
For a group of sequences that you cannot align to each other, you will want to think hard about what sorts of evolutionary questions you want to ask - PAML is not an appropriate tool for that.
Whatever analysis you do with PAML, you always want to start with aligned sequences, not unaligned, and it should always be a meaningful alignment (be careful, because alignment software always gives you some sort of alignment, even for unrelated sequences - it'll just be a bad alignment in that case. same for tree-building tools)
Good luck in your learning,
Janet