Gabriel Portwood
unread,Dec 17, 2025, 6:19:17 PM (2 days ago) Dec 17Sign in to reply to author
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Hello! I am studying the evolutionary origins and ancestral reconstruction of the Transthyretin/HIUase protein family and I have been using PAML 4.10.9 for my analyses. I am studying this protein superfamily that went through a gene duplication event and I was trying to run codeml analyses for the whole tree; however, I have been running into some problems and my supervisor told me to divide my whole tree analysis for now to both a Transthyretin subtree and a HIUase subtree and then run the codeml program for 3x4 and FMutSel, but I am running into the same problems. The following messages are show when I try to run codeml:
BEBing (dim = 4). This may take several minutes.
Calculating f(x_h|w): 10 categories 20 w sets.
Calculating f(X), the marginal likelihood.
log(fX) = -8597.920495 S = -8404.984277 -188.955221
Calculating f(w|X), posterior probabilities of site classes.
Error: edid 158 / 158 patterns
This is happening for both subtrees and the whole tree analyses (just different numbers and values showing up, but the same error)
My supervisor and I don't know how to fix those issues and how to go through our analyses again.
I am sending the files used for the analyses.
Best regards,
Gabe ;)