PAML CODEML running

[Abhishikha Srivastava]

Dear collegues,

I performed a  sites model  analysis in codeml with a bacterial gene but getting result of 

"NG86 unusable"...i m unable to understand what is happening???I enclosed here all my three files codon seq file,tree file,control file

                 Pls.help me

Thanks!!!!!

[cajawe]

The "NG86 unusable" message isn't really important; that section of the output is simply reporting codeml's efforts to generate starting parameter estimates, and codeml can generally run fine despite problems applying the NG86 method.  Your actual problem is on the last line of your "error.png" screenshot; "Species 0.9885?".  Here, codeml has read your treefile and it's trying (but failing) to find a taxon named "0.9885".  This problem stems from your use of a treefile that contains node support values.  You should remove those and try again.  

[Abhishikha Srivastava]

Thanks for your reply!!!!!I got my result

[Vivek Keshri]

I was getting same problem and after removing the  nodes support value, PAML is properly working 

[Vivek Keshri]

Thanks you very much Abhishikha for posting this error. And thanks a lot to Cajawe for great response.. 

Vivek