20 nos seeds were sown on 29th April 2018
These 20 Seedlings were transplanted at 23 DAS in May 2018
The tillers of the 20 seedlings were carefully separated at 22 days by thumb to 103 seedlings that were again transplanted @ 45 DAS (2nd Transplant)
83 survived (80%) that provided 276 seedlings which were further transplanted @ 67 DAS (3rd transplant)
260 survived (94%) that provided 820 seedlings which were finally transplanted @ 90 DAS (4th transplant)
808 seedlings (98%) finally survived that gave 25.12 kg in 46.2 sqm equivalent to 5.44 MT/ha.
The Clonal Propagation Technique was demonstrated by Dr R H Richharia at the Central Rice Research Institute (CRRI) -Cuttack in 1960s...
The journal Nature Vol-194 published on 12th May 1962 has his paper on Clonal Propagation.
In this manner, even a single pure seed can be multiplied by this technique and a normal rice crop can be raised during the following season from seeds obtained from clones which give higher production than yields obtained from seed to seed crop. This is because the grains obtained from clones are fully mature and healthy filled with least percentage of chaffy grains due to the physiological efficiency of tiller plants.
Dr Riccharia had observed an increased yield of 17 to 61% as compared to culture from normal seedlings; these seeds also were more resilient to pests and diseases.
In this manner any promising rice variety in any region can be spread rapidly within a season and yields can be improved.
This method is particularly useful in flood prone areas; older rice seedlings from unaffected plots can be utilised as a source of clones and transplanted. Dr Riccharia had observed that rice clones resist water submergence and thus are effective in flood prone regions.
This technique was successfully taken up in 25 demonstration trials in 10 villages in Cuttack Sadar in 1963.
The results of Clonal Propagation were published in the prestigious science journal - Nature in 1962.