Cell detection tutorial issue

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Austin Theroux

unread,

Jan 20, 2022, 3:05:46 PM1/20/22

to oufti

Hello,

I am using Oufti in MATLAB R2021b on a Macbook Pro. I was following the Cell detection tutorial here and when I got to clicking "This frame" (Step 13 in the guide), I receive a warning dialog that "analysis was not saved due to error: not enough input arguments". In MATLAB, I get the following in the command window:

Decreasing the fsmooth value in the parameters doesn't change the error unless fsmooth is set to 1 or below. In this case, I get a new error in MATLAB:

processing region 1
region 1 discarded, area = 52
processing region 2
mesh not created for region 2 :consider decreasing fsmooth value
processing region 3
mesh not created for region 3 :consider decreasing fsmooth value
processing region 4
mesh not created for region 4 :consider decreasing fsmooth value
processing region 5
region 5 discarded, area = 8
processing region 6
mesh not created for region 6 :consider decreasing fsmooth value
processing region 7
mesh not created for region 7 :consider decreasing fsmooth value
processing region 8
region 8 discarded, area = 8
processing region 9
mesh not created for region 9 :consider decreasing fsmooth value
processing region 10
region 10 discarded, area = 8
processing region 11
mesh not created for region 11 :consider decreasing fsmooth value
processing region 12
mesh not created for region 12 :consider decreasing fsmooth value
processing region 13
mesh not created for region 13 :consider decreasing fsmooth value
processing region 14
mesh not created for region 14 :consider decreasing fsmooth value
processing region 15
mesh not created for region 15 :consider decreasing fsmooth value
processing region 16
mesh not created for region 16 :consider decreasing fsmooth value
processing region 17
mesh not created for region 17 :consider decreasing fsmooth value
processing region 18
mesh not created for region 18 :consider decreasing fsmooth value
processing region 19
mesh not created for region 19 :consider decreasing fsmooth value
processing region 20
mesh not created for region 20 :consider decreasing fsmooth value
processing region 21
mesh not created for region 21 :consider decreasing fsmooth value
processing region 22
mesh not created for region 22 :consider decreasing fsmooth value
processing region 23
mesh not created for region 23 :consider decreasing fsmooth value
processing region 24
region 24 discarded, area = 47
processing region 25
mesh not created for region 25 :consider decreasing fsmooth value
processing region 26
mesh not created for region 26 :consider decreasing fsmooth value
frame 1 finished, elapsed time 1.0646 s

processing region 1
region 1 discarded, area = 52
processing region 2
mesh not created for region 2 :consider decreasing fsmooth value
processing region 3
mesh not created for region 3 :consider decreasing fsmooth value
processing region 4
mesh not created for region 4 :consider decreasing fsmooth value
processing region 5
region 5 discarded, area = 8
processing region 6
mesh not created for region 6 :consider decreasing fsmooth value
processing region 7
mesh not created for region 7 :consider decreasing fsmooth value
processing region 8
region 8 discarded, area = 8
processing region 9
mesh not created for region 9 :consider decreasing fsmooth value
processing region 10
region 10 discarded, area = 8
processing region 11
mesh not created for region 11 :consider decreasing fsmooth value
processing region 12
mesh not created for region 12 :consider decreasing fsmooth value
processing region 13
mesh not created for region 13 :consider decreasing fsmooth value
processing region 14
mesh not created for region 14 :consider decreasing fsmooth value
processing region 15
mesh not created for region 15 :consider decreasing fsmooth value
processing region 16
mesh not created for region 16 :consider decreasing fsmooth value
processing region 17
mesh not created for region 17 :consider decreasing fsmooth value
processing region 18
mesh not created for region 18 :consider decreasing fsmooth value
processing region 19
mesh not created for region 19 :consider decreasing fsmooth value
processing region 20
mesh not created for region 20 :consider decreasing fsmooth value
processing region 21
mesh not created for region 21 :consider decreasing fsmooth value
processing region 22
mesh not created for region 22 :consider decreasing fsmooth value
processing region 23
mesh not created for region 23 :consider decreasing fsmooth value
processing region 24
region 24 discarded, area = 47
processing region 25
mesh not created for region 25 :consider decreasing fsmooth value
processing region 26
mesh not created for region 26 :consider decreasing fsmooth value
frame 1 finished, elapsed time 0.92526 s

processing region 1
region 1 discarded, area = 52
processing region 2
fitquality aligning = 0
fitting region 2 - quality check failed - try resplitting (1)
region 2 splitted and the number of regions increased - return for test
processing region 2
fitquality aligning = 0
fitting region 2 - quality check failed - try resplitting (2)
region 2 splitted - return for test
processing region 2
fitquality aligning = 0
fitting region 2 - quality check failed - try resplitting (3)
region 2 splitted - return for test
processing region 2
fitquality aligning = 0
fitting region 2 - quality check failed - try resplitting (4)
region 2 splitted - return for test
processing region 2
fitquality aligning = 0
fitting region 2 - quality check failed - no cell found
processing region 3
fitquality aligning = 0
fitting region 3 - quality check failed - try resplitting (1)
region 3 splitted - return for test
processing region 3
fitquality aligning = 0
fitting region 3 - quality check failed - try resplitting (2)
region 3 splitted - return for test
processing region 3
fitquality aligning = 0
fitting region 3 - quality check failed - try resplitting (3)
region 3 splitted - return for test
processing region 3
fitquality aligning = 0
fitting region 3 - quality check failed - try resplitting (4)
region 3 splitted - return for test
processing region 3
fitquality aligning = 0
fitting region 3 - quality check failed - no cell found
processing region 4
fitquality aligning = 0
fitting region 4 - quality check failed - try resplitting (1)
region 4 splitted - return for test
processing region 4
fitquality aligning = 0
fitting region 4 - quality check failed - try resplitting (2)
region 4 splitted - return for test
processing region 4
fitquality aligning = 0
fitting region 4 - quality check failed - try resplitting (3)
region 4 splitted - return for test
processing region 4
fitquality aligning = 0
fitting region 4 - quality check failed - try resplitting (4)
region 4 splitted - return for test
processing region 4
fitquality aligning = 0
fitting region 4 - quality check failed - no cell found
processing region 5
region 5 discarded, area = 8
processing region 6
fitquality aligning = 0
fitting region 6 - quality check failed - try resplitting (1)
region 6 splitted - return for test
processing region 6
fitquality aligning = 0
fitting region 6 - quality check failed - try resplitting (2)
region 6 splitted - return for test
processing region 6
fitquality aligning = 0
fitting region 6 - quality check failed - try resplitting (3)
region 6 splitted - return for test
processing region 6
fitquality aligning = 0
fitting region 6 - quality check failed - try resplitting (4)
region 6 splitted - return for test
processing region 6
fitquality aligning = 0
fitting region 6 - quality check failed - no cell found
processing region 7
fitquality aligning = 0
fitting region 7 - quality check failed - try resplitting (1)
region 7 splitted - return for test
processing region 7
fitquality aligning = 0
fitting region 7 - quality check failed - try resplitting (2)
region 7 splitted - return for test
processing region 7
fitquality aligning = 0
fitting region 7 - quality check failed - try resplitting (3)
region 7 splitted - return for test
processing region 7
fitquality aligning = 0
fitting region 7 - quality check failed - try resplitting (4)
region 7 splitted - return for test
processing region 7
fitquality aligning = 0
fitting region 7 - quality check failed - no cell found
processing region 8
region 8 discarded, area = 8
processing region 9
fitquality aligning = 0
fitting region 9 - quality check failed - try resplitting (1)
region 9 splitted - return for test
processing region 9
fitquality aligning = 0
fitting region 9 - quality check failed - try resplitting (2)
region 9 splitted - return for test
processing region 9
fitquality aligning = 0
fitting region 9 - quality check failed - try resplitting (3)
region 9 splitted - return for test
processing region 9
fitquality aligning = 0
fitting region 9 - quality check failed - try resplitting (4)
region 9 splitted - return for test
processing region 9
fitquality aligning = 0
fitting region 9 - quality check failed - no cell found
processing region 10
region 10 discarded, area = 8
processing region 11
fitquality aligning = 0
fitting region 11 - quality check failed - try resplitting (1)
region 11 splitted - return for test
processing region 11
fitquality aligning = 0
fitting region 11 - quality check failed - try resplitting (2)
region 11 splitted - return for test
processing region 11
fitquality aligning = 0
fitting region 11 - quality check failed - try resplitting (3)
region 11 splitted - return for test
processing region 11
fitquality aligning = 0
fitting region 11 - quality check failed - try resplitting (4)
region 11 splitted - return for test
processing region 11
fitquality aligning = 0
fitting region 11 - quality check failed - no cell found
processing region 12
fitquality aligning = 0
fitting region 12 - quality check failed - try resplitting (1)
region 12 splitted - return for test
processing region 12
fitquality aligning = 0
fitting region 12 - quality check failed - try resplitting (2)
region 12 splitted - return for test
processing region 12
fitquality aligning = 0
fitting region 12 - quality check failed - try resplitting (3)
region 12 splitted - return for test
processing region 12
fitquality aligning = 0
fitting region 12 - quality check failed - try resplitting (4)
region 12 splitted - return for test
processing region 12
fitquality aligning = 0
fitting region 12 - quality check failed - no cell found
processing region 13
fitquality aligning = 0
fitting region 13 - quality check failed - try resplitting (1)
region 13 splitted - return for test
processing region 13
fitquality aligning = 0
fitting region 13 - quality check failed - try resplitting (2)
region 13 splitted - return for test
processing region 13
fitquality aligning = 0
fitting region 13 - quality check failed - try resplitting (3)
region 13 splitted - return for test
processing region 13
fitquality aligning = 0
fitting region 13 - quality check failed - try resplitting (4)
region 13 splitted - return for test
processing region 13
fitquality aligning = 0
fitting region 13 - quality check failed - no cell found
processing region 14
fitquality aligning = 0
fitting region 14 - quality check failed - try resplitting (1)
region 14 splitted and the number of regions increased - return for test
processing region 14
fitquality aligning = 0
fitting region 14 - quality check failed - try resplitting (2)
region 14 splitted - return for test
processing region 14
fitquality aligning = 0
fitting region 14 - quality check failed - try resplitting (3)
region 14 splitted - return for test
processing region 14
fitquality aligning = 0
fitting region 14 - quality check failed - try resplitting (4)
region 14 splitted - return for test
processing region 14
fitquality aligning = 0
fitting region 14 - quality check failed - no cell found
processing region 15
fitquality aligning = 0
fitting region 15 - quality check failed - try resplitting (1)
region 15 splitted - return for test
processing region 15
fitquality aligning = 0
fitting region 15 - quality check failed - try resplitting (2)
region 15 splitted - return for test
processing region 15
fitquality aligning = 0
fitting region 15 - quality check failed - try resplitting (3)
region 15 splitted - return for test
processing region 15
fitquality aligning = 0
fitting region 15 - quality check failed - try resplitting (4)
region 15 splitted - return for test
processing region 15
fitquality aligning = 0
fitting region 15 - quality check failed - no cell found
processing region 16
fitquality aligning = 0
fitting region 16 - quality check failed - try resplitting (1)
region 16 splitted - return for test
processing region 16
fitquality aligning = 0
fitting region 16 - quality check failed - try resplitting (2)
region 16 splitted - return for test
processing region 16
fitquality aligning = 0
fitting region 16 - quality check failed - try resplitting (3)
region 16 splitted - return for test
processing region 16
fitquality aligning = 0
fitting region 16 - quality check failed - try resplitting (4)
region 16 splitted - return for test
processing region 16
fitquality aligning = 0
fitting region 16 - quality check failed - no cell found
processing region 17
fitquality aligning = 0
fitting region 17 - quality check failed - try resplitting (1)
region 17 splitted - return for test
processing region 17
fitquality aligning = 0
fitting region 17 - quality check failed - try resplitting (2)
region 17 splitted - return for test
processing region 17
fitquality aligning = 0
fitting region 17 - quality check failed - try resplitting (3)
region 17 splitted - return for test
processing region 17
fitquality aligning = 0
fitting region 17 - quality check failed - try resplitting (4)
region 17 splitted - return for test
processing region 17
fitquality aligning = 0
fitting region 17 - quality check failed - no cell found
processing region 18
fitquality aligning = 0
fitting region 18 - quality check failed - try resplitting (1)
region 18 splitted and the number of regions increased - return for test
processing region 18
fitquality aligning = 0
fitting region 18 - quality check failed - try resplitting (2)
region 18 splitted - return for test
processing region 18
fitquality aligning = 0
fitting region 18 - quality check failed - try resplitting (3)
region 18 splitted - return for test
processing region 18
fitquality aligning = 0
fitting region 18 - quality check failed - try resplitting (4)
region 18 splitted - return for test
processing region 18
fitquality aligning = 0
fitting region 18 - quality check failed - no cell found
processing region 19
fitquality aligning = 0
fitting region 19 - quality check failed - try resplitting (1)
region 19 splitted - return for test
processing region 19
fitquality aligning = 0
fitting region 19 - quality check failed - try resplitting (2)
region 19 splitted - return for test
processing region 19
fitquality aligning = 0
fitting region 19 - quality check failed - try resplitting (3)
region 19 splitted - return for test
processing region 19
fitquality aligning = 0
fitting region 19 - quality check failed - try resplitting (4)
region 19 splitted - return for test
processing region 19
fitquality aligning = 0
fitting region 19 - quality check failed - no cell found
processing region 20
fitquality aligning = 0
fitting region 20 - quality check failed - try resplitting (1)
region 20 splitted - return for test
processing region 20
fitquality aligning = 0
fitting region 20 - quality check failed - try resplitting (2)
region 20 splitted - return for test
processing region 20
fitquality aligning = 0
fitting region 20 - quality check failed - try resplitting (3)
region 20 splitted - return for test
processing region 20
fitquality aligning = 0
fitting region 20 - quality check failed - try resplitting (4)
region 20 splitted - return for test
processing region 20
fitquality aligning = 0
fitting region 20 - quality check failed - no cell found
processing region 21
fitquality aligning = 0
fitting region 21 - quality check failed - try resplitting (1)
region 21 splitted - return for test
processing region 21
fitquality aligning = 0
fitting region 21 - quality check failed - try resplitting (2)
region 21 splitted - return for test
processing region 21
fitquality aligning = 0
fitting region 21 - quality check failed - try resplitting (3)
region 21 splitted - return for test
processing region 21
fitquality aligning = 0
fitting region 21 - quality check failed - try resplitting (4)
region 21 splitted - return for test
processing region 21
fitquality aligning = 0
fitting region 21 - quality check failed - no cell found
processing region 22
fitquality aligning = 0
fitting region 22 - quality check failed - try resplitting (1)
region 22 splitted - return for test
processing region 22
fitquality aligning = 0
fitting region 22 - quality check failed - try resplitting (2)
region 22 splitted - return for test
processing region 22
fitquality aligning = 0
fitting region 22 - quality check failed - try resplitting (3)
region 22 splitted - return for test
processing region 22
fitquality aligning = 0
fitting region 22 - quality check failed - try resplitting (4)
region 22 splitted - return for test
processing region 22
fitquality aligning = 0
fitting region 22 - quality check failed - no cell found
processing region 23
fitquality aligning = 0
fitting region 23 - quality check failed - try resplitting (1)
region 23 splitted - return for test
processing region 23
fitquality aligning = 0
fitting region 23 - quality check failed - try resplitting (2)
region 23 splitted - return for test
processing region 23
fitquality aligning = 0
fitting region 23 - quality check failed - try resplitting (3)
region 23 splitted - return for test
processing region 23
fitquality aligning = 0
fitting region 23 - quality check failed - try resplitting (4)
region 23 splitted - return for test
processing region 23
fitquality aligning = 0
fitting region 23 - quality check failed - no cell found
processing region 24
region 24 discarded, area = 47
processing region 25
fitquality aligning = 0
fitting region 25 - quality check failed - try resplitting (1)
region 25 splitted - return for test
processing region 25
fitquality aligning = 0
fitting region 25 - quality check failed - try resplitting (2)
region 25 splitted - return for test
processing region 25
fitquality aligning = 0
fitting region 25 - quality check failed - try resplitting (3)
region 25 splitted - return for test
processing region 25
fitquality aligning = 0
fitting region 25 - quality check failed - try resplitting (4)
region 25 splitted - return for test
processing region 25
fitquality aligning = 0
fitting region 25 - quality check failed - no cell found
processing region 26
fitquality aligning = 0
fitting region 26 - quality check failed - try resplitting (1)
region 26 splitted - return for test
processing region 26
fitquality aligning = 0
fitting region 26 - quality check failed - try resplitting (2)
region 26 splitted - return for test
processing region 26
fitquality aligning = 0
fitting region 26 - quality check failed - try resplitting (3)
region 26 splitted - return for test
processing region 26
fitquality aligning = 0
fitting region 26 - quality check failed - try resplitting (4)
region 26 splitted - return for test
processing region 26
fitquality aligning = 0
fitting region 26 - quality check failed - no cell found
processing region 27
region 27 discarded, area =
processing region 28
region 28 discarded, area =
processing region 29
fitquality aligning = 0
fitting region 29 - quality check failed - try resplitting (1)
region 29 splitted - return for test
processing region 29
fitquality aligning = 0
fitting region 29 - quality check failed - try resplitting (2)
region 29 splitted - return for test
processing region 29
fitquality aligning = 0
fitting region 29 - quality check failed - try resplitting (3)
region 29 splitted - return for test
processing region 29
fitquality aligning = 0
fitting region 29 - quality check failed - try resplitting (4)
region 29 splitted - return for test
processing region 29
fitquality aligning = 0
fitting region 29 - quality check failed - no cell found
processing region 30
region 30 discarded, area =
processing region 31
region 31 discarded, area =
processing region 32
fitquality aligning = 0
fitting region 32 - quality check failed - try resplitting (1)
region 32 splitted - return for test
processing region 32
fitquality aligning = 0
fitting region 32 - quality check failed - try resplitting (2)
region 32 splitted - return for test
processing region 32
fitquality aligning = 0
fitting region 32 - quality check failed - try resplitting (3)
region 32 splitted - return for test
processing region 32
fitquality aligning = 0
fitting region 32 - quality check failed - try resplitting (4)
region 32 splitted - return for test
processing region 32
fitquality aligning = 0
fitting region 32 - quality check failed - no cell found
processing region 33
region 33 discarded, area =
processing region 34
region 34 discarded, area =
processing region 35
fitquality aligning = 0
fitting region 35 - quality check failed - try resplitting (1)
region 35 splitted - return for test
processing region 35
fitquality aligning = 0
fitting region 35 - quality check failed - try resplitting (2)
region 35 splitted - return for test
processing region 35
fitquality aligning = 0
fitting region 35 - quality check failed - try resplitting (3)
region 35 splitted - return for test
processing region 35
fitquality aligning = 0
fitting region 35 - quality check failed - try resplitting (4)
region 35 splitted - return for test
processing region 35
fitquality aligning = 0
fitting region 35 - quality check failed - no cell found
frame 1 finished, elapsed time 4.9795 s

Thank you for any help provided!

Not enough input arguments (Warning dialog)

Austin Theroux

unread,

Jan 20, 2022, 3:07:24 PM1/20/22

to oufti

Correction: After decreasing fsmooth to 1 or below, the MATLAB errors are only the "quality check failed - try resplitting" type. I accidentally copy and pasted previous error messages.

Oufti

unread,

Jan 21, 2022, 5:14:48 AM1/21/22

to oufti

Hi Austin,

Hard to tell what exactly is going on, a couple of ideas/fixes:

-Try reloading the parameters and make sure you cell good segmentation results when you click the Segmentation button (Step 10.2-12 in the tutorial).

-Oufti is typically only compatible with Matlab up to 2013/2014, so this could still be a compatibility issue. Perhaps try running it in an earlier version of Matlab.

-Also, keep in mind that we have had many issues with Oufti on Mac computers after OS upgrades and have stopped supporting Oufti on Apple products. You can have a look at other forum questions and will quickly find out that, unfortunately, you are not the only Mac user with Oufti issues.

Hope this helps somewhat, thanks for using Oufti!

Oufti-team

Op donderdag 20 januari 2022 om 21:07:24 UTC+1 schreef austin....@yale.edu:

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