Tracking Rotation

[Nico]

Dear Oufti team,

I was happy to have found MicrobeTracker and subsequently Oufti today, and firstly wanted to thank you for your work!!

I need to track rotational motion of magnetized e coli in magnetic fields in order to assess their magnetic moment. I was wondering whether its possible to quantify angular velocity with oufti?

Best,

Nico

[Oufti]

Dear Nico,

You should be able to detect your cells in consecutive frames using Oufti.  My recommendation to get the segmentation working would be to review the parameters and their descriptions in the Oufti web page and focus on the segmentation module to get the best segmented image possible. From there, it might be most helpful to play with the parameters on the provided example data sets and see how the cell meshes are affected. In general, I would adjust one parameter at a time and go from there. Unfortunately, we are unable to tune parameters for all users.

Once your segmentation is complete, you should be able to use the information stored in Oufti's cellList data structure (https://oufti.org/application.htm#js-outputFormat) to calculate angular velocities yourself (as this option is not available within Oufti).

Thanks for using Oufti!

Oufti-team

Op woensdag 26 oktober 2022 om 15:02:40 UTC+2 schreef cybe...@gmail.com: