issue with adjusting contrast

[Kamilla]

Hi everyone,

I am having issues with adding my fluorescent channels. The phase contrast image appears alright, however when I load my fluorescent channel images, they seem to lose some information. Specifically, when I try to adjust the contrast setting to fix the image, the data range is changed to 0-1. How can I fix that? I have attached the screenshot of what I see after I upload the images, with how it's supposed to look like

[Oufti]

Hi Kamilla,

This is because the image is normalized when loaded into Oufti. Please see https://groups.google.com/g/ouftiinfo/c/OHSg1lw9P-E/m/DQrBNpaUCgAJ for more details.

Hope this helps!

Op donderdag 14 maart 2024 om 15:30:46 UTC+1 schreef Kamilla: