Hi Pablo,
No, it isn't possible to add in test routes into the live Darwin service. RDG do provide a staging service where it is possible to do this but it is only available to TOCs or to companies providing services to TOCs. As you mention a train PIS, if you are working for a TOC then they can request access for you to the Darwin staging service.
Please note that there is a specific service, Darwin4Trains, which is designed to support train PIS. You may want to investigate gaining access to this service, if you haven't already, as it may save you some time and effort.
Andrew