Hi Alex,
Thank you for your help, it can be calibrated correctly now.
I have a new problem with tracking.
After clicking the tracking without display, the commands says multimed_r_nlay stopped after 40 iterations and it seems that it does not link the particle between frames.
Do you have any idea what is the possible reason for this?
I have two other basic questions:
1. where do we define the frame per second we used in experiment (the time difference between two frames)?
2. what is the unit of tracking parameters? I think it defines the maximum search windows for the same particle between two frames. Is the unit mm?
Thank you for your time to check my problems.
Best Regards
Jiang