Frog sperm tracking
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Pedro Aguiar
Mar 5, 2025, 11:41:20 AMMar 5
to OpenCASA Mailing List
Hello all,
Has anyone worked with frog sperm tracking analyses? Frog sperm typically have very elongated heads, and I suspect this might affect tracking accuracy in CASA software. I’d appreciate any advice on optimizing settings or choosing the best methods to ensure reliable tracking for amphibian sperm.
Has anyone worked with frog sperm tracking analyses? Frog sperm typically have very elongated heads, and I suspect this might affect tracking accuracy in CASA software. I’d appreciate any advice on optimizing settings or choosing the best methods to ensure reliable tracking for amphibian sperm.
f.martin...@gmail.com
Mar 5, 2025, 11:46:00 AMMar 5
to Pedro Aguiar, OpenCASA Mailing List
Hi Pedro,
We have worked with chicken sperm (also, elongated) and no problem.
Yes, frog sperm could be tricky, but apparently it is not a big deal
if you have good contrast and the tails are not affecting the
detection. If you have problems with that, you might send an example
video and we might help with that.
As a trick, if you open the video in ImageJ and save it as AVI with
PNG compression, it becomes considerably smaller with no loss of
quality... even JPEG is acceptable and it can be <2 MB (I added this
to the group welcome).
Best regards,
Felipe
El mié, 5 mar 2025 a las 16:41, Pedro Aguiar (<pagui...@gmail.com>) escribió:
>
> Hello all,
>
> Has anyone worked with frog sperm tracking analyses? Frog sperm typically have very elongated heads, and I suspect this might affect tracking accuracy in CASA software. I’d appreciate any advice on optimizing settings or choosing the best methods to ensure reliable tracking for amphibian sperm.
>
> --
> You received this message because you are subscribed to the Google Groups "OpenCASA Mailing List" group.
> To unsubscribe from this group and stop receiving emails from it, send an email to opencasa_mailing...@googlegroups.com.
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We have worked with chicken sperm (also, elongated) and no problem.
Yes, frog sperm could be tricky, but apparently it is not a big deal
if you have good contrast and the tails are not affecting the
detection. If you have problems with that, you might send an example
video and we might help with that.
As a trick, if you open the video in ImageJ and save it as AVI with
PNG compression, it becomes considerably smaller with no loss of
quality... even JPEG is acceptable and it can be <2 MB (I added this
to the group welcome).
Best regards,
Felipe
El mié, 5 mar 2025 a las 16:41, Pedro Aguiar (<pagui...@gmail.com>) escribió:
>
> Hello all,
>
> Has anyone worked with frog sperm tracking analyses? Frog sperm typically have very elongated heads, and I suspect this might affect tracking accuracy in CASA software. I’d appreciate any advice on optimizing settings or choosing the best methods to ensure reliable tracking for amphibian sperm.
>
> You received this message because you are subscribed to the Google Groups "OpenCASA Mailing List" group.
> To unsubscribe from this group and stop receiving emails from it, send an email to opencasa_mailing...@googlegroups.com.
> To view this discussion, visit https://groups.google.com/d/msgid/opencasa_mailinglist/38310c48-0b23-4ab1-9134-ae2072298a55n%40googlegroups.com.
Pedro Aguiar
Mar 5, 2025, 11:55:59 AMMar 5
to OpenCASA Mailing List
Thanks a lot for the suggestions, Felipe!
Currently, I do not have access to samples, but I plan to start collecting them at the end of this year.
Would it be possible for you to send me one or two videos of chicken sperm so I can visualize them on CASA? It would really help with my trials.
Currently, I do not have access to samples, but I plan to start collecting them at the end of this year.
Would it be possible for you to send me one or two videos of chicken sperm so I can visualize them on CASA? It would really help with my trials.
Best,
Pedro
Felipe Martínez Pastor
Mar 6, 2025, 2:05:36 PMMar 6
to opencasa_m...@googlegroups.com
Hi Pedro,
You can find the video and the image with the configuration in this link:
https://we.tl/t-wmTVLzSkSd
The video is only 20 MB because I used JPEG compression (it might take
several seconds to load while decompressing).
Not very good motiltiy (thawed semen), but OpenCASA can detect the tracks.
Best wishes,
Felipe
El 5/3/25 a las 17:55, Pedro Aguiar escribió:
You can find the video and the image with the configuration in this link:
https://we.tl/t-wmTVLzSkSd
The video is only 20 MB because I used JPEG compression (it might take
several seconds to load while decompressing).
Not very good motiltiy (thawed semen), but OpenCASA can detect the tracks.
Best wishes,
Felipe
El 5/3/25 a las 17:55, Pedro Aguiar escribió:
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