Welcome to the OpenCASA mailing group. All members are welcome to collaborate.
If you find any problem or bug, please describe it with all possible detail (please, read below WELL KNOWN PROBLEM). Provide information on your configuration:
- Operative system, processor and memory.
- ImageJ or Fiji version.
- OpenCASA version.
- Any error messages.
- Microscope model, type of optics (describe your working objective), camera model, acquisition software.
- Video size, frame rate, scale (microns/pixel). A complete collection of your settings, if possible (especially, min/max area, min. track length, and max. displacement).
You can share a representative video, but try to keep it of minimal length to reproduce your problem.
Behave kindly and be patient.
WELL-KNOWN PROBLEM:
OpenCASA v. 2 does not work if directly installed on recent versions of ImageJ/Fiji. This is the "NA error." If you try analyzing, you only get NA in the results window.

You need to go to https://wsr.imagej.net/distros/, go to the folder for your system, and download an older version (1.53k from 2021 should work). Just unzip 1.53k, run it, install OpenCASA (do not forget javaml-0.1.7.jar), and run it. Close it and go back to your modern version (maybe it is a good idea to reinstall the plugin).

You might want to check if Java is up to date on your computer. If it's not working, try switching between zips, including Java or "nojre" (no Java).

Mussel Sperm

Shannen Keyser