Issue related to ImageJ and Video for Open CASA
Dhiraj Bhimrao Puri
Hello,
I hope you all are doing well. My name is Dhiraj Puri, and I am a research scholar from BITS Pilani, Goa Campus, India. I am currently working on human sperm cells and need to use the Open CASA plugin in ImageJ for tracking.
I have tried different versions of ImageJ (1.54, 1.53, and 1.49), but I am unable to track the sperm cells successfully. I would appreciate your help in determining whether the issue lies with the video or the plugin.
Here is the link to the video: https://drive.google.com/file/d/1n7q_FepMQGRAKM7I5h8rOx-0rV95DRmF/view?usp=sharing
Thank you for your time and assistance. Looking forward to your quick response.
Best regards,
Dhiraj Puri
Department of Mechanical Engineering
Joaquín Gadea
I have evaluated the video you shared, and I can detect the sperm motility in OpenCasa (although I do not have the exact scale of your microscope system to calaculate the microns per pixel, I used 0.2).
Based on my experience with my MSc students, I think you have a problem with the ImageJ version.
Best wishes.
Joaquin
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Joaquín Gadea
Dear Dhiraj Puri,
I have not proccesed the video. It was used directly.
The key points are:
the settings (mainly the microns/pixel and number of frames rate of your camera). In this example I use 0.28 microns/pixel and 50 frames/sec.
the quality of the video,
good luck
Joaquin
El 18/02/2025 a las 5:55, Dhiraj Bhimrao Puri escribió:
Thank you for your response. For my microscope, the micron per pixel is 0.28. I have tried using the video I previously shared, and while I am not encountering the NaN error, I am seeing tracking of irregularities other than sperm cells. Are you processing the video before using it in OpenCAsA (such as adjusting thresholds or other settings)?
To view this discussion, visit https://groups.google.com/d/msgid/opencasa_mailinglist/9a6de860-44be-4217-b0be-ae16324fa2b1%40um.es.
The information contained in this electronic communication is intended solely for the individual(s) or entity to which it is addressed. It may contain proprietary, confidential and/or legally privileged information. Any review, retransmission, dissemination, printing, copying or other use of, or taking any action in reliance on the contents of this information by person(s) or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this communication in error, please notify us by responding to this email or telephone and immediately and permanently delete all copies of this message and any attachments from your system(s). The contents of this message do not necessarily represent the views or policies of BITS Pilani.
-- Prof. Joaquín Gadea DVM, PhD, Dipl. ECAR Full Professor in Physiology (Catedrático de Universidad) Dept. Fisiologia. Facultad de Veterinaria Universidad de Murcia-Murcia 30.100-Spain Phone 34 868 884655 e-mail: jga...@um.es www.um.es/fisiorep