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Welcome to the OpenCASA mailing group. All members are welcome to collaborate.
If you find any problem or bug, please describe it with all possible detail (please, read below WELL KNOWN PROBLEM). Provide information on your configuration:
- Operative system, processor and memory.
- ImageJ or Fiji version.
- OpenCASA version.
- Any error messages.
- Microscope model, type of optics (describe your working objective), camera model, acquisition software.
- Video size, frame rate, scale (microns/pixel). A complete collection of your settings, if possible (especially, min/max area, min. track length, and max. displacement).
You can share a representative video, but try to keep it of minimal length to reproduce your problem.
Behave kindly and be patient.
WELL-KNOWN PROBLEM:
OpenCASA v. 2 does not work if directly installed on recent versions of ImageJ/Fiji. This is the "NA error." If you try analyzing, you only get NA in the results window.

You need to go to https://wsr.imagej.net/distros/, go to the folder for your system, and download an older version (1.53k from 2021 should work). Just unzip 1.53k, run it, install OpenCASA (do not forget javaml-0.1.7.jar), and run it. Close it and go back to your modern version (maybe it is a good idea to reinstall the plugin).

You might want to check if Java is up to date on your computer. If it's not working, try switching between zips, including Java or "nojre" (no Java).

0 selected

3/24/20

Output parameters and video manipulation

Hi all, Thank you so much to the creators for this great plugin! I am currently using it to assess

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Output parameters and video manipulation

Hi all, Thank you so much to the creators for this great plugin! I am currently using it to assess

3/24/20

rmeenhasanzade, Felipe Martínez Pastor2

1/20/20

Pre-Processing Input Data

Hi, It seems that you are using positive phase contrast with a higher magnification (20X?). I believe

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Pre-Processing Input Data

Hi, It seems that you are using positive phase contrast with a higher magnification (20X?). I believe

1/20/20

Munish Bedi, Camila Arroyo Salvo3

11/21/19

How to calculate settings for motility analysis of bovine semen

Yes it opens .avi file in my case On Thursday, November 21, 2019 at 1:26:24 AM UTC+5:30, Camila

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How to calculate settings for motility analysis of bovine semen

Yes it opens .avi file in my case On Thursday, November 21, 2019 at 1:26:24 AM UTC+5:30, Camila

11/21/19

View more pinned conversations

Pedro Aguiar, Felipe Martínez Pastor4

Mar 6

Frog sperm tracking

Hi Pedro, You can find the video and the image with the configuration in this link: https://we.tl/t-

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Frog sperm tracking

Hi Pedro, You can find the video and the image with the configuration in this link: https://we.tl/t-

Mar 6

Felipe Martínez-Pastor2

Mar 5

OpenCASA Window Size for high fps, sharing experience and asking for discussion/advice

Hi, Responding to myself, in case it is helpful for others or spurring dicussion. I realised I was

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OpenCASA Window Size for high fps, sharing experience and asking for discussion/advice

Hi, Responding to myself, in case it is helpful for others or spurring dicussion. I realised I was

Mar 5

Sertan Koşan, Felipe Martínez Pastor2

Feb 26

NaN and thresholding 255-255

Hi Sertan, I added the issue to the group presentation. This is a known bug, OpenCASA resists working

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NaN and thresholding 255-255

Hi Sertan, I added the issue to the group presentation. This is a known bug, OpenCASA resists working

Feb 26

Dhiraj Bhimrao Puri, Joaquín Gadea4

Feb 18

Issue related to ImageJ and Video for Open CASA

Thank you all! I have finally been able to track sperm cells. I tested each version available in the

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Issue related to ImageJ and Video for Open CASA

Thank you all! I have finally been able to track sperm cells. I tested each version available in the

Feb 18

f.martin...@gmail.com

Feb 17

OpenCASA mail group welcome message

Hi, I have improved the welcome message. Sharing here to remind everyone how to report errors or ask

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OpenCASA mail group welcome message

Hi, I have improved the welcome message. Sharing here to remind everyone how to report errors or ask

Feb 17

Léa Dasque, … Felipe Martínez-Pastor4

Feb 13

Issue with OpenCASA Returning Only NA Values

Hello, Thank you for your answers. Yes indeed the objective is not negative phase contrast so I

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Issue with OpenCASA Returning Only NA Values

Hello, Thank you for your answers. Yes indeed the objective is not negative phase contrast so I

Feb 13

Carlos Alquezar, … rosperez8

Feb 8

OpenCASA for Educational purposes

Hi! I am delighted to know that OpenCASA is being used for educational purposes in many universities.

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OpenCASA for Educational purposes

Hi! I am delighted to know that OpenCASA is being used for educational purposes in many universities.

Feb 8

Alejandro FILI, f.martin...@gmail.com2

Feb 7

Video files and presets

Hi Alejandro. First of all, you have find a well-known glitch in OpenCASA. You need to go to ImageJ

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Video files and presets

Hi Alejandro. First of all, you have find a well-known glitch in OpenCASA. You need to go to ImageJ

Feb 7

Diego Moreno, … Felipe Martínez Pastor4

Jan 10

OpenCASA on Mac M series

Hi, No idea by now. We need someone proficient with Java and ImageJ workings to solve this problem.

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OpenCASA on Mac M series

Hi, No idea by now. We need someone proficient with Java and ImageJ workings to solve this problem.

Jan 10

Erick José Ramo Da Silva, Felipe Martínez-Pastor2

10/30/24

Reducing the size of video files using ffmpeg

Hi Erick, I understand that you had color videos and you could make them smaller by removing the

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Reducing the size of video files using ffmpeg

Hi Erick, I understand that you had color videos and you could make them smaller by removing the

10/30/24

4/30/24

openCASA parameters for rat sperm

Hello openCASA team, I am wondering if anyone has used the openCASA system for rat sperm, do you mind

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openCASA parameters for rat sperm

Hello openCASA team, I am wondering if anyone has used the openCASA system for rat sperm, do you mind

4/30/24

Pilar Ameijeiras, Felipe Martínez-Pastor4

3/26/24

Tracking human sperm (second round)

Hi, All seems good with the parameters (did you check the scale, anyway?). Just for testing, you

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Tracking human sperm (second round)

Hi, All seems good with the parameters (did you check the scale, anyway?). Just for testing, you

3/26/24

Felipe Martínez-Pastor

12/6/23

Some reminder of OpenCASA 2 glitches and workarounds (to be hopefully solved in the future)

Hi, Just a reminder that OpenCASA 2 could give you some problems if installed with the last ImageJ

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Some reminder of OpenCASA 2 glitches and workarounds (to be hopefully solved in the future)

Hi, Just a reminder that OpenCASA 2 could give you some problems if installed with the last ImageJ

12/6/23

Felipe Martínez Pastor, … jyaniz7

10/26/23

Issue with OpenCASA settings -- max. displacement units

Thank you Carlos. I was also planning to explore the code (no idea of Java, but I believe I can

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Issue with OpenCASA settings -- max. displacement units

Thank you Carlos. I was also planning to explore the code (no idea of Java, but I believe I can

10/26/23

genji grace, … Felipe Martínez Pastor14

10/25/23

Motility analysis returning "NAN",too!

Hi, It is enough if you enter that in the OpenCASA settings. However, you should use imageJ to

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Motility analysis returning "NAN",too!

Hi, It is enough if you enter that in the OpenCASA settings. However, you should use imageJ to

10/25/23

Carlos Alquezar, f.martin...@gmail.com2

10/12/23

Some thoughts about the OpenCASA Community and future directions

Thank you Carlos, you sure did a great work! I totally agree with your idea. Indeed, anyone with

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Some thoughts about the OpenCASA Community and future directions

Thank you Carlos, you sure did a great work! I totally agree with your idea. Indeed, anyone with

10/12/23

Felipe Martínez Pastor, Erick José Ramo Da Silva3

10/8/23

Tip for storing uncompressed AVI videos

Hi, Did you use a lossless algorithm? The idea behind using 7zip was not altering the original files

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Tip for storing uncompressed AVI videos

Hi, Did you use a lossless algorithm? The idea behind using 7zip was not altering the original files

10/8/23

Felipe Martínez Pastor, Daniel Salvador3

10/8/23

Update: OpenCASA 2 working fine in last ImageJ and Fiji

Great! I'm so glad you could solve the issue. Not sure if we could add it to the OpenCASA Github

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Update: OpenCASA 2 working fine in last ImageJ and Fiji

Great! I'm so glad you could solve the issue. Not sure if we could add it to the OpenCASA Github

10/8/23

K Rockefeller, … Daniel Salvador4

10/7/23

Motility analysis returning "NaN"

Thanks Felipe for your help. I forgot to introduce myself in my first post. I am a Med Vet from

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Motility analysis returning "NaN"

Thanks Felipe for your help. I forgot to introduce myself in my first post. I am a Med Vet from

10/7/23

Pilar Ameijeiras, … Felipe Martínez Pastor5

10/6/23

Tracking human sperm

Hi everyone, I managed to solve my problem! Like you said before it was a problem with the version of

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Tracking human sperm

Hi everyone, I managed to solve my problem! Like you said before it was a problem with the version of

10/6/23

6/23/23

success cases with positive phase contrast?

First I'd like to thank the creator of this open source software. I also want to ask whether

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success cases with positive phase contrast?

First I'd like to thank the creator of this open source software. I also want to ask whether

6/23/23

debarun roy, … jyaniz5

6/21/23

OpenCASA for Marmoset sperm

Hi Debarun, try this link: http://wsr.imagej.net/distros/win/ij150-win-java8.zip All the best, Jesus

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OpenCASA for Marmoset sperm

Hi Debarun, try this link: http://wsr.imagej.net/distros/win/ij150-win-java8.zip All the best, Jesus

6/21/23

4/29/23

Hi, I am new to OpenCASA and was wondering if anyone has or can suggest the cut off values

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Hi, I am new to OpenCASA and was wondering if anyone has or can suggest the cut off values

4/29/23

João Santana, … Jesus Yániz27

3/29/23

Not tracking the sperm cells

Hi, You are right Felipe. The OpenCASA was optimized for negative phase contrast microscopy. We also

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Not tracking the sperm cells

Hi, You are right Felipe. The OpenCASA was optimized for negative phase contrast microscopy. We also

3/29/23

Nikita Egorov, jyaniz5

3/11/23

Problem with output window

Dear Nikita, We also used the official version of ImageJ with standard settings, I don't think

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Problem with output window

Dear Nikita, We also used the official version of ImageJ with standard settings, I don't think

3/11/23

natalia oscoz, … Will Skinner3

3/9/23

Recording human sperm- Calculate Hyperactivation

Hi Natalia, In humans, the hyperactivity cutoff values should be VCL ≥150 μm/s AND LIN ≤50% AND ALH ≥

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Recording human sperm- Calculate Hyperactivation

Hi Natalia, In humans, the hyperactivity cutoff values should be VCL ≥150 μm/s AND LIN ≤50% AND ALH ≥

3/9/23

AlfaVeta Oxie, jyaniz2

2/3/23

Settings for consentration module with canine semen.

Dear Artur, The images you sent have not enough contrast for a proper analysis. I recomend you use

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Settings for consentration module with canine semen.

Dear Artur, The images you sent have not enough contrast for a proper analysis. I recomend you use

2/3/23

Diego Moreno, jyaniz2

2/3/23

Dear Diego, We have detected a problem of compatibility of the OpenCASA with the last version of the

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Dear Diego, We have detected a problem of compatibility of the OpenCASA with the last version of the

2/3/23

Katherine Hamil, … Felipe Martínez Pastor3

11/24/22

Writing a Macro for mp4 to avi conversion

Hi, There are certain netiquette that we try to follow. Certainly, writing in capitals is considered

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Writing a Macro for mp4 to avi conversion

Hi, There are certain netiquette that we try to follow. Certainly, writing in capitals is considered

11/24/22

JGSMx 99, Felipe Martínez Pastor2

11/23/22

Hi, I'm answering in English, so everybody in the list can follow. Please, do not forget

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Hi, I'm answering in English, so everybody in the list can follow. Please, do not forget

11/23/22

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