Re: Sam Shanmugam Solution Manual Random 19
Arnau Cyr
Materials and methods: Forty age-related cataractous nuclei removed during manual small incision cataract surgery were obtained and randomly immersed in 25 mM lanosterol solution or in control solution and stored at room temperature for 6 days. Pre- and post-immersion photographs were graded by two masked observers and collated for the regression or progression of lens opacity.
Zhao et al. have shown reversal of naturally occurring lenticular opacity by immersing rabbit lenses in lanosterol solution and incubating them for 6 days.[1] We modeled our study on the same lines as Zhao et al., with the basic difference being that we used human senile nuclei obtained by the process of small incision manual cataract surgery instead of intact lenses, thereby lacking in the lens capsule, and cortex. However, we were unable to replicate the results of Zhao et al. in our study using human cataractous nuclei. Lenticular opacification progressed in 90% nuclei in the lanosterol group and in 70% of the control group. The grade of opacification after 6 days of incubation was also not significantly different between the two groups. One of the observers felt that one nucleus appeared less opaque, but this was not supported by the other observer and is too weak a link to rely upon [Fig. 4].