RNA-seq providers and prices

[Kilo Jarek]

Hi everyone,

I am asking for a friend... Let's assume I want to run a small standard RNA-seq experiment (source: human cell lines total RNA, n ~12-24 samples max), to look predominantly on mRNA, but (depending on price) not excluding looking at microRNA and lnRNA. I am interested in comparing drug-treated cell lines with controls to identify differentially expressed genes.

What are the current prices for RNA-seq and what providers/services do you use or can recommend (or charge...)? A price of ~£400/sample including "basic analysis" (which I am not interested in) was floated and that feels extremely expensive to me. But I have never personally ordered RNA-seq so I don't know what's the baseline these days. 

Any recommendations would be very appreciated.

Thanks a lot!

Jarek

[Leo Zeef]

Hi Jarek

It depends ...😊

Roughly:

miRNA is a separate library to mRNA, so double cost versus mRNA alone. 

lnRNA needs rRNA depletion and more sequencing, so double cost versus mRNA alone.

So:

mRNA £200 per sample

lnRNA £400 per sample

mRNA and miRNA £400 per sample

lnRNA and miRNA £600 per sample

bw

Leo

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[Ian Sudbery]

We use Novogene for pretty much everything, and have never had a problem, or, if we have once or twice, then its more than made up for by the lower price, and i've had some failed samples with every sequencing provider I've used. Our last project was 23 days from sample receipt to data release.  I think i'm not supposed to reveal the prices, but I can say that it was substantially less than those quoted by Leo, at least for the mRNA. As pointed out, miRNA is a separate experiment. lncRNA is about 50% extra. The advantage of Novogene over, say BGI, is that standard sequencing is done in the UK, so you don't need to navigate international couriers etc. 

I believe that other commercial providers are attempting to be competitive with Novogene, although I think they struggle. You might try getting a quote from Azenta (what was Genewiz), I know they've been ramping up their UK capacity.  

As part of the UCU industrial action, we are only working our contracted hours. It might take me a bit longer than usual to get back to you and I may decline voluntary responsibilities or opportunities - thank you for your patience. 

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Ian Sudbery

Senior Lecturer in Bioinformatics,

Sheffield Institute for Nucleic Acids,

School of Biosciences,

The University of Sheffield. 

web: www.sudlab.co.uk

Tel: 0114 222 2738

Twitter: IanSudbery

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[Kilo Jarek]

Thank you both very much, this is good news already. And anybody please keep the prices/providers coming :-)

Jarek