'no vienna value set' error

[Jonesy Jones]

Hiya,

I am trying to check out the RNA Peak Generator. I uploaded a NOESY spectra, an RNA sequence, and selected only imino corresponding atoms, but I got a 'no vienna value set' message when I tried to run the program.

Do I have to generate a secondary structure of my RNA first, assuming that's what the vienna error corresponds to? Or is there some other parameter I've accidentally omitted somewhere?

Thanks,

Jonesy

[janm]

Hi Jonesy, one way to give NvJ the info it wants is as follows:

Assign - Atoms

Then: Reference - Predict - RNA using Sec. Structure...

In the window that appears give your predicted secondary structure in Vienna format and hit Predict.

Bruce has a tutorial video called "RNA Prediction and Network Slider" which may be useful, found here:

http://www.onemoonscientific.com/nmrviewj/tutorials

The interface used to define the nuclei of interest has changed since this was recorded, hopefully the current version is self-explanatory.

Cheers,

Jan