Display of NUS spectrum

[Jun Zhang]

Dear Bruce,

    

     Recently, I collected some 2D N15 TROSY HSQC spectra by nonuniform sampling (NUS). I processed the data using NMRpipe IST model and the spectrum is displayed normal by nmrDraw. Then I use the following script to convert the spectrum into NMRviewJ:

#!/bin/csh
nmrPipe -in exact.ft2 -verb    \
| pipe2xyz -out nustest.nv -nv

The script is the one I always used and never have any problem.

When I used NMRviewJ (9.2.0-b2), the spectrum can be displayed, but contour level shown in Attributes panel is 0.000. When I adjust the bar, I can change the contour level, but the number is still 0. Also, when I tried to overlay the spectrum with the other spectrum that was collected uniform sampling, the NUS spectrum disappears. Could you tell me what could cause the problem?

Thanks!

Jun

 

[brucej]

Hi Jun,

Every dataset has a scale value.  I've typically had this set at 1.0e6.  When reading values from the dataset they are divided by this value.  I'm guessing that this dataset needs to have the value set differently (for example, changed from 1.0e6 to 1.0).  You can see the value in the Datasets Table Appearance section.  Change the value and then write out the dataset .par file so it is saved for that dataset.

Bruce

[Jun Zhang]

Hi Bruce,

  It works. You are right. The level is just very small. Thank you for the help.

  I am wondering whether in future NMRviewJ will have a module to analyze cpmg relaxation data. It seems the software has everything it need to move one more step to have this feature.

Jun

[brucej]

Glad it worked!

News about CPMG (and more) coming soon.

Part of http://comdnmr.nysbc.org (of which I'm a Co-PI)

Bruce