Generating Constraints from NOE data

[kye stachowski]

Hey all,

I am currently working on obtaining distance restraints for a molecule I am working on using NOE data. I have assigned all of the peaks in the spectrum and I am trying build a constraint table. I am currently stuck in the process as nothing happens when I click "Extract Unambiguous" on the NOE peak lists. Since the constraint table is not being populated, I dont think I can bin the distance until I do this.

I have attached a screenshot of eveything, maybe I have something setup incorrectly. 

Your help is appreciated!

Thank you,

Kye Stachowski

[kye stachowski]

I assume that this is why i keep getting this error when I go to use CalcMed for the calibration step in obtaining these restraints.