NMRPipe To Sparky Pipeline

[Brandon Guerra]

Hi all - I am trying to learn to use sparky to do protein backbone assignments, specifically to assign signals on a 15N/1H HSQC but find myself unable to produce the right files from the processed 3D data sets to open the HN slices on sparky. Overall I would appreciate a good resource to learn SPARKY independently. - Thank you!

[Lee, Woonghee]

You can use xyz2pipe from NMRPipe to create a single file from multiple 2D planes. Then, pipe2ucsf or drag and drop in POKY.

Woonghee Lee, I.E.I.P., M.S., Ph.D.

Assistant Professor & Data Science and Chemistry Certificate Program Director
Department of Chemistry
University of Colorado Denver
1151 Arapahoe St. (Science Bldg.) Rm 4128A (office), Rm 4128 (dry lab), Rm 4133 (wet lab)
Denver, CO 80217-3364, USA
Office: +1-303-315-7672

woongh...@ucdenver.edu

https://poky.clas.ucdenver.edu
https://poky.clas.ucdenver.edu/wlee-group
https://clas.ucdenver.edu/chemistry/woonghee-lee
https://clas.ucdenver.edu/chemistry/data-science-and-chemistry-certificate

Shipping/Mailing Address:
Woonghee Lee
1201 5th St. UCD CHEM-194
P.O. Box 173364 (USPS)
Denver, CO 80204, USA

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From: nmr-s...@googlegroups.com <nmr-s...@googlegroups.com> on behalf of Brandon Guerra <brandong...@gmail.com>
Sent: Sunday, November 2, 2025 11:50:00 AM
To: NMR POKY/SPARKY USER GROUP <nmr-s...@googlegroups.com>
Subject: [NMR POKY/SPARKY] NMRPipe To Sparky Pipeline

 

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Hi all - I am trying to learn to use sparky to do protein backbone assignments, specifically to assign signals on a 15N/1H HSQC but find myself unable to produce the right files from the processed 3D data sets to open the HN slices on sparky. Overall I would appreciate a good resource to learn SPARKY independently. - Thank you!

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