Axis synchronization error?

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Moriah Beck

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Aug 28, 2025, 6:14:14 PM (7 days ago) Aug 28
to NMR POKY/SPARKY USER GROUP
When I use APES with series of backbone experiments, I don't get any peaks for one spectra (hncacb) and it looks like the axis are different than the other spectra.  I tried to use [yt] to synchronize, but can't figure out how to get peaks picked.  See screenshots in attached document.
Ig4_peakpickingprob.docx

Lee, Woonghee

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Aug 28, 2025, 6:19:40 PM (7 days ago) Aug 28
to Moriah Beck, NMR POKY/SPARKY USER GROUP

Hi, I am afraid if the problem stems from different causes, but you can try to change spectrum axis order. In the POKY Notepad “Pn”, go to File -> POKY Scripts -> Find Additional …. Then search for “change_spectrum_axis.py” and run by cmd+b (ctrl+b). You will be able to create a new spectrum with the updated axis order. Good luck!

 

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From: nmr-s...@googlegroups.com <nmr-s...@googlegroups.com> on behalf of Moriah Beck <moria...@gmail.com>
Date: Thursday, August 28, 2025 at 4:14 PM
To: NMR POKY/SPARKY USER GROUP <nmr-s...@googlegroups.com>
Subject: [NMR POKY/SPARKY] Axis synchronization error?

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When I use APES with series of backbone experiments, I don't get any peaks for one spectra (hncacb) and it looks like the axis are different than the other spectra.  I tried to use [yt] to synchronize, but can't figure out how to get peaks picked.  See screenshots in attached document.

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