Definition of 5' - 3' region in the case of BED file with locations of varying length

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Shmuel

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Jan 8, 2020, 9:07:46 AM1/8/20
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Hello,
I am plotting (multiple) files using locations from a bed file.The locations in the bed file are of varying length (300-4000). What is the definition of the  5'-3' region in such a case? For example, will it be the location with the maximum length? Is it documented somewhere?
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