Electrical stim.data streams cannot be recorded correctly.

[Xuan LUO]

Hi NR users,

We are using NeuroRighter to deliver a real-time closed-loop systems. During the experiment,  we are trying to use SqueakySpk to process data streams that we recorded and have met several problems.

version: NeuroRighter v1.1.0.564

DAQ card: 3 pieces of PCIe 6259

system: windows 7

Matlab code from: downloads section of the NR google code site and SqueakySpk matlab class( we have noticed that the loadspike.m code cannot be used to read .spk file that we got, we are using loadspk.m).

The electrical stim.data streams cannot record stimulation time and channels.

Experiment procedure:

1).Ensure hardware settings;

2).Test impedance;

3).Set recording streams;

4).Use manual stimulation to deliver defined stimulation to chosen channel( we can see the correct waveform through oscilloscope, and we can see stimulation artifact in spikes GUI)

5). Create a new file and enable recording, hit the green start button. Start!

6).The problem is coming: the .stim file that we recorded is always 22 bytes. No matter how long we recorded.

7).Use Matlab to read .spk and .stim files;

The result:

NEURORIGHTER SPIKE RECORD

Sampling rate (Hz): 25000

ADC Polling period (sec): 0.05

Number of channels: 64

Digital gain: 2

This file does not contain unit information

Samples per waveform: 50

Recording time (yr-mo-dy-hr-mi-sc-ms): 2016-5-20-21-42-9-227

Number of spikes: 19488

recording duration: 67.9025

------------

Entering search for desired spikes.

Looking for first spike... done

Looking for last spike... done

Loading a total of 19488 spikes: 

Starting at time 0.00056

Ending at time 67.9025

...done

Load complete.

NEURORIGHTER STIMULATION RECORD

Sampling rate: 25000

Recording time (yr-mo-dy-hr-mi-sc-ms): 2016-5-20-21-42-9-227

Number of stimuli: 0

We can see that there is no data in .stim file.

Could you please give us some advice about this problem?

Thank you very much!

Xuan LUO

[Xuan LUO]

Attached with the pictures for reference.

[Riley Zeller-Townson]

Well, I can see from your hardware settings tab that you've configured NR to record stimulation data on device 3.  The next question you should ask is whether or not your hardware itself is configured to match the settings you have in software.  Take a look at this part of the user manual: https://sites.google.com/site/neurorighter/user-manual/system-setup#_Toc226960254

From the manual, this corresponds to the connection between AO 3 and AI 16 on device 3.  If that connection is in place already, it would be a good idea to put an oscope on that signal and verify that upon stimulation you get signals there as well. 

On Thu, Jul 14, 2016 at 9:56 AM, Xuan LUO <yob...@outlook.com> wrote:

Attached with the pictures for reference.

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Riley Zeller-Townson

PhD student, Butera lab

Laboratory for Neuroengineering

Georgia Tech/Emory University

Atlanta, GA, USA