Autoimmune hemolytic anemia / tocopherol / oxidation
ironj...@aol.com
Assessment of lipid peroxidation and serum vitamin E concentration in
dogs with immune-mediated hemolytic anemia.
Pesillo SA, Freeman LM, Rush JE.
Department of Clinical Sciences, School of Veterinary Medicine, Tufts
University, North Grafton, MA 01536, USA.
OBJECTIVE: To determine plasma malondialdehyde (MDA) and serum vitamin
E concentrations in dogs with immune-mediated hemolytic anemia (IMHA)
and healthy control dogs. SAMPLE POPULATION: Serum and plasma samples
from 36 dogs with IMHA and 40 healthy control dogs. PROCEDURE: Blood
samples were collected from all study dogs. Plasma MDA concentrations
were measured by use of a commercial colorimetric assay, and serum
vitamin E concentrations (alpha-, gamma, and delta-tocopherol
concentrations) were measured via high-performance liquid
chromatography. RESULTS: Plasma MDA concentrations were significantly
higher in the dogs with IMHA than in the control dogs. Compared with
control dogs, serum alpha-, gamma-, and &tocopherol concentrations were
significantly lower in the IMHA-affected dogs. CONCLUSIONS AND CLINICAL
RELEVANCE: Results indicated a state of oxidative stress and reduced
antioxidant reserve in dogs with IMHA; this finding provides support
for further investigation of the potential benefits of antioxidant
treatment in dogs with this disease.
PMID: 15631024 [PubMed - in process]
--------------------------------------------------------------------------------
Who loves ya.
Tom