What is the difference between Acid-PAGE and SDS-PAGE?

[Sang Heon Kim]

Hello, I am a student studying wheat.

Acid PolyAcryamide Gel Electrophoresis (A-PAGE) and Sodium Dodecyl Sulfate PolyAcryamide Gel Electrophoresis (SDS-PAGE) are used to analyze seed storage proteins.

I wonder three things.

1. Is there any difference between A-PAGE and SDS-PAGE?

2. I can easily find a paper or a protocol using SDS-PAGE but it's hard to find a paper or a protocol using A-PAGE. Is SDS-PAGE better method to analyze seed storage proteins than A-PAGE?

3. If 2nd question is true, then why is A-PAGE still used in some studies? Is there any advantage that A-PAGE have only?

I wonder clamping.

[Rakesh Kumar]

1. Acid PAGE is atype of native PAGE, where  buffer is made of acetic acid and 2M urea. It is used to separate high mol. wt proteins. 

2. There are papers discussing the protocol of Acid page.  Cereal chem 71(3) 238-242.

[Rakesh Kumar]

 Acid page is a type of native page, where buffer is made of acetic acid and 2M urea. It is used to separate high mol. wt proteins.

On Monday, February 3, 2014 12:11:48 PM UTC+5:30, Sang Heon Kim wrote: