mitochondria staining

[ankit sabharwal]

How can I stain mitochondria in specific in fixed cells or zebrafish embryos?

I wish to use a method that doesn't employ any fluorescent stain like FITC or fluorescein because my probe is GFP tagged.

Thanks

[Julia Parnis]

Dear Ankit,

Mitochondrial stains usually involve fluorescently-tagged molecules. However, if you'll choose red-tagged fluorophores, they won't interfere with GFP staining.

It is possible to stain mitochondria via immunocytochemistry. For example, use anti-Cytochrome C Oxidase or anti-TOM20 (stainings that I tried) as primary antibodies and use secondary antibodies conjugated to red fluorophores. Another possibility is prior fixation to use MitoTracker Red CMXRos (Life Technologies), which accumulates as a function of mitochondrial activity and stays after aldehyde-based fixations.

I hope it helps,
Best of luck,
Julia

[Sg A]

Hello,

Please be advised, that Mitotrackers are supposed to be used in living cells, not in fixed ones.

Info on how to observe mitochondrial morphology is available at https://www.thermofisher.com/ru/ru/home/life-science/cell-analysis/cell-structure/mitochondria.html .

(My response is impressed by Scigine (http://www.scigine.com/)