One end cip in blunt ligation
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Tom47865
Jul 4, 2013, 7:23:02 AM7/4/13
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Hi
Im curious have anybody used a strategy where you cip one end of the vector and one end of the insert for blunt end ligation?
To avoid vector background and to avoid wrong way round insert.
Tom
Im curious have anybody used a strategy where you cip one end of the vector and one end of the insert for blunt end ligation?
To avoid vector background and to avoid wrong way round insert.
Tom
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