ChIP of Nf-kB/P65 in MEFs

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muneef ayyash

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Jul 18, 2013, 4:51:05 PM7/18/13
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hi
I am facing some problem doing ChIP of P65 in mouse fibroblasts...

their are many variations between experiments and some times there is no amplification of the
signal checked by qRT-pcr of IL6 promoter

any suggestions? protocol that is working would be of much help

thank you
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