PLGA and TEM

[Marija Petrusevska]

We are working with PLGA nanospheres and trying to obtain TEM images, 

however the obtained images are blurred i.e. like there is no sample on the grids. 

Can you suggest any protocol?

[raju...@gmail.com]

I would like share few suggestions regarding this problem 

1. PLGA is one kind of organic polymer and this type of material has less contrast in side the TEM chamber due to their 

 poor conductivity for this need to use Lacey carbon TEM grid in order to enhance the better contrast.

2. If you want to take better image - Need use 200 kv field emission gun TEM at low resolution.

 Hopefully this will work for all kinds of polymeric materials 

 

With regards 

Poliraju.K