protein precipitation during dialysis

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Ajeet singh

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Oct 6, 2013, 2:37:03 PM10/6/13

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I purified my recombinant His tag protein in Tris,pH 7.5 (pI of protein 8.9) using 400mM Imidazole conc. in elution buffer. I dialysed my protein for removing imidazole but it is being precipitated.

20mM Tris,pH 7.5, 150mM KCl, 5%glycerol, that dialysis buffer composition I used. what to do?

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