Crosslink IP

96 views
Skip to first unread message

c.surridge

unread,
Jun 6, 2013, 9:07:31 AM6/6/13
to nature-protoc...@googlegroups.com

Derya Sargin asks:


I’m looking for a detailed protocol for crosslinking the antibody to the protein A/G agarose beads usingDSS. I would appreciate any feedback. Thanks!

Bronwen

unread,
Jul 5, 2013, 11:54:06 AM7/5/13
to nature-protoc...@googlegroups.com
In this protocol http://www.nature.com/protocolexchange/protocols/2183 there is a method for crosslinking to protein A sepharose beads. Would this work for protein A agarose beads?

muneef ayyash

unread,
Jul 18, 2013, 4:27:03 PM7/18/13
to nature-protoc...@googlegroups.com
hi

I am attaching  our lab protocol for crosslinking

goodluck
Crosslinking_Protocol.docx

muneef ayyash

unread,
Jul 18, 2013, 4:27:23 PM7/18/13
to nature-protoc...@googlegroups.com


On Thursday, June 6, 2013 4:07:31 PM UTC+3, c.surridge wrote:
Reply all
Reply to author
Forward
0 new messages