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Nature Protocols Discussion

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Welcome to our discussion forum!

Whilst this group is administered by the Editors of Nature Protocols we hope it will be a community site with active users posting both questions and answers to methodological research problems.

Please remember to ‘tag’ your questions to aid others seeking solutions to problems in your subject area. We hope this forum will grow over the coming months and it will be easier to find answers and browse content if content is tagged appropriately, for example, ‘molecular-biology’, ‘RNAi’, ‘apoptosis’, ‘neuroscience’, ‘MRI’, etc.

Whilst our own interests are in biology and biological chemistry, please do feel free to discuss problems arising in the study of other areas of science.

We look forward to seeing how this group develops and wish you luck in your research.

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Amir Najam Sethit

3/5/18

My Name is : Amir Najam Sethit but google showing error

Please help me out, My name is Amir Najam Sethit but whenever I search my name in a google search, it

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My Name is : Amir Najam Sethit but google showing error

Please help me out, My name is Amir Najam Sethit but whenever I search my name in a google search, it

3/5/18

Babalola Adekunle

8/11/16

Establishment of Stable Cell line

I need help on how to generate a stable neuronal cell line expressing prion protein via pre-

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Establishment of Stable Cell line

I need help on how to generate a stable neuronal cell line expressing prion protein via pre-

8/11/16

5/19/16

A blog about how to search, collect, edit and share research protocols

"Scholarly Research reduces unnecessary experimentation. In fact, people dedicate entire chunks

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A blog about how to search, collect, edit and share research protocols

"Scholarly Research reduces unnecessary experimentation. In fact, people dedicate entire chunks

5/19/16

5/19/16

How to find, collect, edit and share protocols easily and quickly

Dear colleagues, Are you happy with your search for and managing protocols? Please allow me to share

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How to find, collect, edit and share protocols easily and quickly

Dear colleagues, Are you happy with your search for and managing protocols? Please allow me to share

5/19/16

ankit sabharwal, … Sg A3

5/17/16

mitochondria staining

Hello, Please be advised, that Mitotrackers are supposed to be used in living cells, not in fixed

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mitochondria staining

Hello, Please be advised, that Mitotrackers are supposed to be used in living cells, not in fixed

5/17/16

SUPERMAN, Sg A2

5/17/16

MEASURING APOPTOSIS

Hello, Sorry for delay to answer. TUNEL assay is supposed to employed for measuring apoptosis. There

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MEASURING APOPTOSIS

Hello, Sorry for delay to answer. TUNEL assay is supposed to employed for measuring apoptosis. There

5/17/16

Rayees Padder, … Sg A3

5/17/16

Mitochondrial morphology imaging

Hello, Sorry for my late response, maybe it will be helpful for some beginners in the field. Please

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Mitochondrial morphology imaging

Hello, Sorry for my late response, maybe it will be helpful for some beginners in the field. Please

5/17/16

Sebastian Freeman, … Max Roberts4

3/23/16

Microfabrication of organs-on-a-chip protocol

Hi, I was wondering if you every made progress in finding a company which was able to create the

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photolithography

Microfabrication of organs-on-a-chip protocol

Hi, I was wondering if you every made progress in finding a company which was able to create the

3/23/16

1/9/16

Migration and Invasion assays

Hello, I am a PhD student and i have a confusion about how to follow the cancer cell migration and

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Migration and Invasion assays

Hello, I am a PhD student and i have a confusion about how to follow the cancer cell migration and

1/9/16

Katherine Garro

1/9/15

Problems identifying RANTES and TGF-β1 population with the FlowCytomix Pro

Hello, I´ve used the Human Rantes Simplex BMS8287FF Kit and the Human TGF-β1 BMS8249FF Kit in

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Problems identifying RANTES and TGF-β1 population with the FlowCytomix Pro

Hello, I´ve used the Human Rantes Simplex BMS8287FF Kit and the Human TGF-β1 BMS8249FF Kit in

1/9/15

Elamurugan Appavoo

12/25/14

how to confirm a eukaryotically expressed protein?

I am expressing a eukaryotic protein...to confirm the expression of the protein i have transfected

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eukaryoticexpression

invitrotranslation

westernblotting

how to confirm a eukaryotically expressed protein?

I am expressing a eukaryotic protein...to confirm the expression of the protein i have transfected

12/25/14

Marija Petrusevska, raju...@gmail.com2

4/5/14

I would like share few suggestions regarding this problem 1. PLGA is one kind of organic polymer and

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I would like share few suggestions regarding this problem 1. PLGA is one kind of organic polymer and

4/5/14

c.surridge, … laxman poudel4

3/23/14

mesenchymal stem cell

Dear Manoj, As the mesenchymal cells can differentiate into other cell types, you can use them to

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mesenchymal stem cell

Dear Manoj, As the mesenchymal cells can differentiate into other cell types, you can use them to

3/23/14

Sang Heon Kim, Rakesh Kumar3

2/7/14

What is the difference between Acid-PAGE and SDS-PAGE?

Acid page is a type of native page, where buffer is made of acetic acid and 2M urea. It is used to

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What is the difference between Acid-PAGE and SDS-PAGE?

Acid page is a type of native page, where buffer is made of acetic acid and 2M urea. It is used to

2/7/14

Vikash Bhardwaj, c.surridge2

11/14/13

Parallel DNA synthesis

Vikash, This looks like an interesting approach. It might be good to upload a detailed Protocol for

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Parallel DNA synthesis

Vikash, This looks like an interesting approach. It might be good to upload a detailed Protocol for

11/14/13

Alivia, c.surridge4

11/5/13

Live cell imaging in mouse embryonic stem cells

I hope we can find you an answer. I don't know that this is quite what you want but this Protocol

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Live cell imaging in mouse embryonic stem cells

I hope we can find you an answer. I don't know that this is quite what you want but this Protocol

11/5/13

10/6/13

protein precipitation during dialysis

I purified my recombinant His tag protein in Tris,pH 7.5 (pI of protein 8.9) using 400mM Imidazole

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protein precipitation during dialysis

I purified my recombinant His tag protein in Tris,pH 7.5 (pI of protein 8.9) using 400mM Imidazole

10/6/13

9/18/13

Congujation Protocol?

Someone knows about a bacterial conjugation protocol? I just found confuse protocols that are not

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Congujation Protocol?

Someone knows about a bacterial conjugation protocol? I just found confuse protocols that are not

9/18/13

Nilesh Meghani, Stephen Roper4

8/12/13

Protocol for in vivo bioorthogonal click chemistry by Ac4MaNAz

Dear Dr. Roper, I understood that. Thank you for your time. Kind Regards, Nilesh On Wednesday, August

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Protocol for in vivo bioorthogonal click chemistry by Ac4MaNAz

Dear Dr. Roper, I understood that. Thank you for your time. Kind Regards, Nilesh On Wednesday, August

8/12/13

Nilesh Meghani, Nature Protocols Discussion3

7/24/13

Protocol for Flow Cytometry

Dear. Thank you for your response and time. It can be of little help to me but i can't open this

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Protocol for Flow Cytometry

Dear. Thank you for your response and time. It can be of little help to me but i can't open this

7/24/13

7/18/13

ChIP of Nf-kB/P65 in MEFs

hi I am facing some problem doing ChIP of P65 in mouse fibroblasts... their are many variations

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ChIP of Nf-kB/P65 in MEFs

hi I am facing some problem doing ChIP of P65 in mouse fibroblasts... their are many variations

7/18/13

c.surridge, … muneef ayyash4

7/18/13

On Thursday, June 6, 2013 4:07:31 PM UTC+3, c.surridge wrote: Derya Sargin asks: I'm looking for

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On Thursday, June 6, 2013 4:07:31 PM UTC+3, c.surridge wrote: Derya Sargin asks: I'm looking for

7/18/13

jem...@utulsa.edu

7/18/13

Algal DNA extraction

Has anyone worked with nanoplankton? If so, what protocol do you use to extract DNA? I have some very

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Algal DNA extraction

Has anyone worked with nanoplankton? If so, what protocol do you use to extract DNA? I have some very

7/18/13

c.surridge, Dishant Sharma4

7/5/13

PCR Troubleshooting

If you have a predicted sequence and the result after sequencing going to be actual sequence. Try to

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PCR Troubleshooting

If you have a predicted sequence and the result after sequencing going to be actual sequence. Try to

7/5/13

7/5/13

Purification of Fatty Acids using SPE

Hi community, I was thinking of using SPE cartidges to purify preparative quantities of fatty acids

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Free_Fatty_Acids

Purification of Fatty Acids using SPE

Hi community, I was thinking of using SPE cartidges to purify preparative quantities of fatty acids

7/5/13

7/4/13

One end cip in blunt ligation

Hi Im curious have anybody used a strategy where you cip one end of the vector and one end of the

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One end cip in blunt ligation

Hi Im curious have anybody used a strategy where you cip one end of the vector and one end of the

7/4/13

Themos Vasilopoulos, Maxwell Peterson2

6/28/13

PCR fusion issue

I was once stuck for days on a tough PCR, but it worked when we varied the temperature during the

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PCR fusion issue

I was once stuck for days on a tough PCR, but it worked when we varied the temperature during the

6/28/13

6/17/13

Detection of large protein molecules

Kellis Rolland 16 Jun 2013 | 12:38 Thank's, very informative!

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Detection of large protein molecules

Kellis Rolland 16 Jun 2013 | 12:38 Thank's, very informative!

6/17/13

6/6/13

Expression of protein with pI 8.2

Elizabeth McEwen 09 Mar 2012 | 08:07 Thanks for your valuable input Dave. Cannot say about the OP but

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Expression of protein with pI 8.2

Elizabeth McEwen 09 Mar 2012 | 08:07 Thanks for your valuable input Dave. Cannot say about the OP but

6/6/13

6/6/13

Electrophoresis of DNA (80-200 base pairs)

B Ping 04 May 2012 | 15:00 Hi We use polyacrylamide gels at work on a regular basis (TBE Gels 6%)

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Electrophoresis of DNA (80-200 base pairs)

B Ping 04 May 2012 | 15:00 Hi We use polyacrylamide gels at work on a regular basis (TBE Gels 6%)

6/6/13

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