ChrisNagy,
From the <a href="
http://www.ni.com/pdf/manuals/373649h.pdf" target="_blank">PXI-2529 specifications</a>, it explains that the channel to channel voltage difference, as well as the channel to ground difference, cannot excede 150V. Polarity is not an issue here because we are dealing with the difference between lines. Make sure to take both channel to channel and channel to ground specifications into account or arcing can occur. Also, keep in mind that the channel to channel difference specification applies to all lines on the board and not just the lines being routed. Hope this helps! :smileyhappy: