Meat One Dha Lahore

[Francesca Cruiz]

Duringan episode of ARY News Programme Sham-e-Ramzan, Punjab Food Authority Director General Muhammad Asim Javed disclosed the seizing of 80 kilograms of expired meat from a market in Lahore over the past two days.

"Today (Sunday), 2500 kilograms of expired meat has also been confiscated in a joint operation by the Punjab Food Authority and Food Department from a renowned meat market near Mughulpura," said Muhammad Asim Javed, ARY News reported.

To combat this issue, meat safety teams comprising veterinary doctors and food safety officers are conducting regular operations and surveys in markets to ensure the provision of hygienic meat to citizens, ARY News reported.

Complete traceability along the chain from "Farm to Fork" is guaranteed, with production to the highest standards. We offer a wide portfolio of meat products. Our team of highly skilled experienced staff carefully hand pick and trim each cut to exacting standards, to deliver the ultimate eating experience.

Certified to halal standards, Zenith has been a pioneer in the supply of healthy and fresh meat. It is a trendsetter in ensuring careful and precise livestock selection and slaying of animals and cutting and supply of meat. It is equipped with food safety skills and knowledge appropriate to meat handling activities. As a leading meat and poultry processor, Zenith ensures that its plant premises, fixtures, things, equipment and transport vehicles are cleaned and sanitized regularly.

To be a preferred world-class meat processor through continuous process innovation and investment to deliver safe and consistent quality products to meet and exceed consumer expectations for a sustainable long-term relationship.

To be a preferred world-class meat processor through continuous process innovation and investment to deliver safe and consistent quality products to meet and exceed consumer expectations for a sustainable long term relationship.

Meat Dukan is a registered trademark (brand) of SR Foods (Pvt) Ltd. Established in 2011, Meat Dukan is one of the top tier brands in meat retail industry of Pakistan with one vision to provide the highest quality hygienic meat to our customers. We are taking butcher industry to next level with substantial experience in the field of retail butcher industry.

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Foodborne diseases (FBDs) are a persistent threat to global health. Approximately one in ten people get infected with contaminated food, and 33 million lives are lost each year around the world1. Salmonella enterica is a widespread zoonotic foodborne pathogen which is a serious hindrance to socioeconomic progress globally mainly in developing countries2. The infections caused by S. enterica continue to plague both developed and developing countries but frequently result in high mortality mostly in developing countries. Some of its serovars, such as S. enteritidis, S. typhimurium, S. typhi, and S. paratyphi, are the cause of serious health problems in humans3. The animal-derived foods are the most common carriers of FBDs caused by S. enterica4. The infection occurs by consuming contaminated poultry meat, mutton, beef, and pork. The raw meat becomes contaminated at different stages during animal skinning, slaughtering, evisceration, transport, and meat cutting in butcher shops at local markets2.

Being a tropical country, the ambient temperature in Pakistan remains favorable for the growth of food spoilage bacteria, which makes the meat processed in unhygienic conditions unsafe for human intake. Goat, sheep, cow, buffalo, camel, and poultry meat are usually consumed as a protein diet in the country5. Some factors are responsible for the transmission of Salmonella in humans, such as cross-contamination during meat processing, poor hygiene practices of butchers, improper storage of meat at markets, intake of undercooked or raw meat, insufficient refrigeration and reheating of prepared meat at homes, and a long gap between cooking and eating meat. Due to the ubiquitous nature of Salmonella, its control in food is difficult. Various serotypes of Salmonella have been found in food-producing animals, and many of them are usually excreted in the feces of apparently healthy animal6,7.

In animal husbandry, the increased use of antibiotics for growth promotion, therapeutics, prophylaxis, and metaphylaxis has led to the emergence of antibiotic resistance in commensal, opportunistic, and normal flora of food-producing animals. The vigorous and continuous use of antibiotics in an environment generates a selection pressure that helps the antibiotic-resistant bacteria to survive and access the food chain. Contaminated raw meat is the main source of antibiotic-resistant S. enterica infections in humans3. Many antibiotic-resistant serotypes of Salmonella are notorious for disturbing the food chain1. Another serious concern for public health is the transmission of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Salmonella through the consumption of contaminated food. Several studies have reported the isolation of MDR Salmonella strains that were also resistant to clinically important antibiotics such as Fluoroquinolones, third-generation Cephalosporins, and Carbapenems, which is now considered as an emerging issue around the world2,6.

In order to monitor and control antimicrobial resistance (AMR), many developed countries use a regular AMR monitoring and surveillance system, for instance, National Antimicrobial Resistance Monitoring Systems (NARMS) in the United States8. On the other hand, due to the inadequacy of surveillance networks, accurate diagnostics, and laboratory capacity, there is limited monitoring and surveillance system in developing countries, such as Pakistan9,10. Over the last few years, several studies conducted in Pakistan were focused on checking the prevalence and antibiotic resistance of Salmonella in raw poultry meat11, however, limited data is available for raw mutton and beef2. Also, a few studies have reported the detection and incidence of antibiotic resistance genes in Salmonella isolated from raw meat in Pakistan. In this context, the present study was designed to evaluate the prevalence, antibiotic resistance pattern, and screening resistance genes in Salmonella serovars recovered from raw (mutton, beef, and poultry) meat samples collected from different markets in Lahore, Pakistan.

Different serovars of Salmonella can possess a number of different antibiotic-resistant genes. However, data about the detection of resistance genes in Salmonella isolated from raw meat is rare in Pakistan. In this study, three antibiotic resistance genes were detected in Salmonella strains recovered from raw meat. Given the PCR results, the blaTEM-1 gene was detected in all isolated resistant strains (100%), which is supported by Li et al.16 in their study by detecting a 100% prevalence of blaTEM-1 gene. The prevalence of the catA1 gene in all resistant Salmonella strains was 63.6% in our study, predominantly detected in S. typhimurium (Fig. 4). As compared to our study, the prevalence is low (47.5%) in the findings of Li et al.34. On the other hand, the gyrA gene was detected in only XDR Salmonella strains with a prevalence of 18.1% (Table S3, Fig. 4), in accordance with a previous study that detected the gyrA gene in 20% of Salmonella isolates35. The whole genome sequence (WGS) analysis, specifically the ANI values of the representative MDR-PH8 and XDR-PG2 strains up to 99.96% and 99.98% respectively with the reference Salmonella enterica serovar Typhi further confirmed the identity of these strains as Salmonella enterica serovar Typhi. The genome size of both the sequenced strains and GC content up to 52% is typically in the range for Gram negative bacteria and the members of enterobacteriaceae. Similarly the genome annotations, the number of CDS and subsystems details generally resemble to that of the Salmonella strains, which helped to confirm the identity of the isolated strains in this study as Salmonella enterica serovar Typhi. Overall this study provides comprehensive and useful data regarding the antibiotic resistance status of Salmonella isolated from raw meat in Lahore.

Each of the sample was processed in three steps, i.e. pre-enrichment, selective enrichment, and isolation on selective media. For pre-enrichment, 10 g of each raw meat sample was homogenized and aseptically transferred into a flask containing 90 ml sterile Buffered Peptone Water (BPW, Oxoid CM1049) as a pre-enrichment media and incubated at 37 C for 24 h. After overnight incubation, aliquots from pre-enrichment were transferred into a selective enrichment liquid medium at a ratio of 1:10, i.e. 1 ml of the cultured BPW was inoculated into each test tube containing 10 ml of Selenite-Cysteine Broth (Oxoid CM0699). The tubes were incubated at 37 C for 24 h. The next day, a loopful from Selenite-Cystine Broth was sub-cultured onto Salmonella-Shigella (SS) agar (Liofilchem 610042), XLT-4 agar (Oxoid CM1061), and MacConkey agar (Oxoid CM0007) by using the streak plate method. These plates were incubated overnight at 37 C. After incubation, the colonies with desired characteristics were selected and re-cultured to purify the bacteria from mixed cultures. The purified isolates were also cultured on blood agar (Oxoid CM0055).

The colorless colonies with black centers on SS agar, black or red colonies with a black center on XLT-4 agar, colorless colonies on MacConkey agar, and white/grey non-swarming growth on blood agar were supposed to be Salmonella (as shown in Fig. S.1). The presumptive colonies of Salmonella were then analyzed by gram staining and biochemical characterization, which included the catalase test, oxidase test, indole test, Triple Sugar Iron (TSI) test, Sulfide Indole Motility (SIM) test, MR/VP tests, urease test, citrate utilization test, and phenylalanine deaminase test by following the protocols of International Organization for Standardization36.

3a8082e126