Problems using Musite

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ker8...@gmail.com

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Apr 29, 2013, 3:13:21 PM4/29/13
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I just installed Musite on my Mac (OS X 10.8.3; Java version 7/update 21) and so far I have not been able to get it to locate any phosphorylation sites. The download and installation with the installation wizard appeared to be successful and the Musite application runs and appears to be functioning properly. However, when I selected the model file H.sapiens.general.ser.thr.tyr and entered the FASTA sequence for human p53 (shown as an example in the Musite publication, Jianjiong Gao, Jay J. Thelen, A. Keith Dunker, and Dong Xu. Musite, a Tool for Global Prediction of General and Kinase-Specific Phosphorylation Sites. Molecular & Cellular Proteomics. 2010. 9(12):2586-600), no phosphorylation sites were found. I got the message "Failed to classify using model H.sapiens-General-Ser and Thr" (and the same thing for the Tyr model). Do you have any suggestions?

Thank you!
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