Détection rapide de Muscardin (ou autre micromammfière) par extraction d'ADN issu.... d'urine !

[Guillaume Marchais]

https://www.researchgate.net/publication/349242289_Quick_detection_of_a_rare_species_Forensic_swabs_of_survey_tubes_for_hazel_dormouse_Muscardinus_avellanarius_urine

[...] Like other species of rodent, the hazel dormouse excretes urine freely and this was highlighted by ultraviolet torch, swabbed from the paper, extracted and hazel dormouse eDNA amplified by quantitative polymerase chain reaction (qPCR). Hazel dormouse presence was confirmed in this way in three out of 50 tubes within eight days. Detection by conventional nest survey occurred on day 63 when a hazel dormouse nest was found in a single survey tube. We calculate that amplification of eDNA left behind in tubes increased survey efficiency here at least 12‐fold. Synthesis and applications. In this study we demonstrate that eDNA swabbed from a clean substrate placed in survey apparatus can significantly hasten the detection of a rare species. This method has the potential to broaden the application of eDNA to other terrestrial vertebrates, including surveys at large spatiotemporal scales. Beyond presence/absence, the non‐invasive DNA sample could also offer insights into sex ratio, abundance, behaviour and population genetics.

Guillaume Marchais