DIANNtoMSstatsFormat - Error: cannot allocate vector of size 1.4 Gb

[Maithreyan Kuppusamy]

Hi,

I am running the latest version of MSstatsConvert. I get a " cannot allocate vector size" error.

How can I resolve it?

Here is the code

"diann <- DIANNtoMSstatsFormat( + dianninput, + annotation = annot, + global_qvalue_cutoff = 0.01, + qvalue_cutoff = 0.01, + pg_qvalue_cutoff = 0.01, + useUniquePeptide = TRUE, + removeFewMeasurements = TRUE, + removeOxidationMpeptides = TRUE, + removeProtein_with1Feature = TRUE, + use_log_file = TRUE, + append = FALSE, + verbose = TRUE, + log_file_path = NULL, + MBR = TRUE, + quantificationColumn = "auto")

INFO [2026-02-16 21:25:09] ** Raw data from DIANN imported successfully. Processed 15311479 groups out of 15311479. 100% done. Time elapsed: 1296s. ETA: 0s.. 

Error: cannot allocate vector of size 1.4 Gb"

Thanks,

Maithreyan

[Anthony Wu]

Hi Maithreyan,

A few questions:

- What is the size of your RAM on your computer in GB?

- What is the size of your dataset in GB?

There might be a problem with an inefficient way of how we handle the new DIANN 2.0 format, but want to confirm it's not a problem with your computer running out of memory.

Thanks,

Tony