Standards with MSstats

[Christian Potts]

Hello MSstats team,

I was with you during May Institute last month and would like clarification on what you shared about using standards with MSstats. I got the impression that the globalstandards normalization was the most robust option for normalization. We're conducting a DIA experiment on some mouse tissue samples, and I'm not sure whether I should add standards. In the lectures, it was shared that there are no commercial standards for experiments on the proteome scale (1000s of proteins), and "hybrid proteome" or in-house mixes are used. What do those refer to? Could you please share some wisdom to help me understand?

Thank you for the MSstats help! 
Christian Potts