Nerve Full Movie Download In Italian Hd
Jenette Bregantini
Pamela Imperadore, Dario Parazzoli, Amanda Oldani, Michael Duebbert, Ansgar Büschges, Graziano Fiorito; From injury to full repair: nerve regeneration and functional recovery in the common octopus, Octopus vulgaris. J Exp Biol 1 October 2019; 222 (19): jeb209965. doi:
As a starting point of the study, we unravelled the transcriptional changes that occur at the NMJ upon a rapidly reversible injury to the MAT. To do so, we choose to profile the transcriptome of NMJs in the mouse soleus: indeed, slow-twitch muscles (like the soleus) display a higher degree of plasticity and remodelling capability than fast-twitch muscles (e.g. the EDL) in response to denervation, and are less vulnerable to aging and neurodegenerative conditions [15,16,17]. We determined the transcriptional profiling of soleus NMJs during the time-course of nerve terminal degeneration and regeneration induced by the pore-forming toxin α-Latrotoxin (α-LTx). This neurotoxin specifically targets the MAT causing an acute, calcium-dependent degeneration that is fully reversible, with complete recovery within a few days in mice [18,19,20]. The process of MAT degeneration/regeneration by α-LTx is highly reproducible and can be temporally assessed by imaging the progressive disappearance/reappearance of pre-synaptic markers, and through quantitative electrophysiological analyses of neurotransmission [20, 21]. Upon damage, neurons produce hydrogen peroxide (H2O2), which acts as a major activator of Schwann cells (SCs) both in vivo and in vitro; this manifests as profound morphological changes that allow the clearing of nerve debris by phagocytosis, and the engagement of the pro-regenerative ERK pathway through phosphorylation [20, 22,23,24]. Therefore, to identify genes most critical to regeneration of the NMJ, we intersected the differentially expressed genes (DEGs) caused by α-LTx with our previously published data set of genes activated by H2O2 in SCs [23].
Several signaling pathways converge on Ctgf transcription, including the transcription factors TEADs (Hippo signaling effectors) with their co-activators and Hippo transducers YAP/TAZ [50]. Given the emerging importance of mechanosignaling for SC biology during development and in nerve regeneration, and in light of the fact that TEAD4 is upregulated in the NMJ transcriptome (Additional file 1: Fig. S3D), we looked at YAP expression and localization in response to H2O2 both in vivo and in vitro (Fig. 9). In the adult sciatic nerve, YAP expression in SCs is innervation-dependent (Fig. 9A upper panels and panel B); indeed, YAP disappears from denervated SCs, but reappears in SC nuclei during axonal regeneration [51]. To determine whether H2O2 generated at the lesion site impacts the YAP signal in SC nuclei, we performed pre-crush intra-sciatic nerve injections of catalase and assessed YAP localization three and four days post-injury. YAP is still present in SCs nuclei three days after crush, when neurons start degenerating, and disappears the day after. Catalase treatment caused YAP to disappear from SCs already on day three after damage (Fig. 9A), suggesting that H2O2 sustains YAP transcriptional activity, which in turn drives Ctgf expression. Finally, we cultured SCs and exposed them to H2O2 in the presence of VT107, a pan-TEAD autopalmitoylation inhibitor. VT107 treatment dampened the H2O2-mediated increase in Ctgf levels (Fig. 9C, D), suggesting that TEAD transcription factors are required to fully activate Ctgf expression.
Peripheral nerves are able to regenerate in response to injury; however, the molecular events underlying this process remain to be fully resolved. To further our understanding into this crucial mechanism, we employed a range of in vitro and in vivo approaches that identified key roles for H2O2 and Ctgf in nerve regeneration. We found that in response to axon injury, mitochondria rapidly generate H2O2 to activate surrounding SCs. The local synthesis and release of H2O2 induce extensive transcriptional changes at the injured NMJ, particularly in genes encoding for ECM components, including the YAP-dependent Ctgf up-regulation. Ctgf contributes to functional recovery upon proximal and distal nerve damage by assisting SC migration and, in turn, allowing the oriented re-growth of the axons. The major findings of the present study are summarized in Fig. 10.
(A) Autograft; (B) nerve repaired with hollow chitosan conduit; (C) nerve repaired with MIT. White arrowheads indicate the regenerated nerves; inside the hollow tube the regenerated nerve is clearly visible, while inside MIT the regenerated nerve is not observable, because the conduit is still full of muscle tissue. MIT: Muscle-in-tube.
Where to Watch NerveNerve is available to watch, stream, download and buy on demand at Google Play. Some platforms allow you to rent Nerve for a limited time or purchase the movie and download it to your device.
The data that we provide represents proof-of-concept evidence that peripheral nerves can be stimulated from within the blood vessels using a mm-sized wireless implant. Additional work is needed to develop this technology into a biomedical device for clinical use. For one, hermetically sealed packaging will be needed for chronic implantation of the device. Although thin-film packaging solutions have yet to be fully developed for clinical use, other wireless implants have shown that glass or ceramic casings can enable chronic operation49. Fortunately, the magnetic fields should easily penetrate these materials and thus they are not expected to degrade the power coupling efficiency. Long-term deployment of future endovascular bioelectronics may also require adjunctive therapies using blood thinning pharmaceuticals. Several factors that involve the implantation of devices within the vasculature can promote thrombosis; however, improved techniques along with antithrombotic regimens have been shown to decrease any catastrophic thrombosis due to stent implantations to
Obviously, not only the biochemical, but also the biophysical and mechanical cues, provided by the biomaterial used for peripheral nerve reconstruction have to be carefully concerned in healthy and diabetic conditions when tissue engineering approaches should lead to an optimized remodeling of the physiological conditions provided by autologous nerve grafts. Chitosan, however, represents a valuable biomaterial for future research as it has clearly demonstrated also significant immunomodulatory effects.
MULTIDISCIPLINARY COLLABORATION FOR ITALIAN EXCELLENCE","displayParagraph":"true","bodyContent":"Silvia OLIVI M.D., physiatrist, Head of the MRI Specialist Unit of the Institute of Montecatone, has been collaborating for many years with Carlo SACCO M.D., neurosurgeon of our SSD of Peripheral Nervous System Surgery, for the selection of quadriplegic patients who can benefit from the particular surgical technique of NERVE TRANSFER to the upper limbs to recover the use of the hands. In addition to selection based on clinical conditions, the most appropriate period for surgical treatment should also be evaluated. The results of the surgery can be seen after about a year, while waiting the patient will be followed and subjected to adequate physiotherapy.
","displayLink":"false","uniqueId":"ituol2WSRcg20oP","description":"assets","linkUrl":"none","target":"none","action":"none","scale":"original","imageWidth":"auto","alt":"","title":"assets","align":"widget-align-left","paragraphType":"div"},"className":"contentgroup","widget":,"uniqueId":"ituol2WSRcg20oP","parentUniqueId":"pi4a8fc4rxd20LR"},"page-zones__main-widgets__5e8395107a9a1-widgets__5e83951087ca3-zones__5e8395108a77c-widgets__5e8395108ee04":"ref":19259006,"uniqueHTMLId":"page-zones__main-widgets__5e8395107a9a1-widgets__5e83951087ca3-zones__5e8395108a77c-widgets__5e8395108ee04","name":"5e8395108ee04","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.button","data":"action":"external","text":"CLICK HERE TO EXIT THIS WEB SITE AND TO ACCESS THE WEB SITE OF THE INSTITUTE OF MONTECATONE, REHABILITATION HOSPITAL OF IMOLA (BO), WHERE THE NEWS, IN ITALIAN, WERE REPORTED","uniqueId":"l1b0ko0fHh7GKsQ","target":"_self","url":"http:\/\/www.montecatone.com\/flex\/cm\/pages\/ServeBLOB.php\/L\/IT\/IDPagina\/959","align":"widget-align-left","fileName":"","className":"button","widget":,"uniqueId":"l1b0ko0fHh7GKsQ","parentUniqueId":"pi4a8fc4rxd20LR","page-zones__main-widgets__5e8391d0b43f7":"ref":19258983,"uniqueHTMLId":"page-zones__main-widgets__5e8391d0b43f7","name":"5e8391d0b43f7","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.row","data":"layout":"youtube-2a","rowType":"youtube-2a","backgroundType":"color","height":"default","horizontalAlignment":"center","width":"contained","style":"light","verticalAlignment":"center","originalRowTemplateId":"youtube_2a","uniqueId":"24M33ffWNaPiMQt","animationEffect":"fade","shapeDivider":"none","shapeDividerColor":"accent","className":"row","widget":,"uniqueId":"24M33ffWNaPiMQt","parentUniqueId":"3lEwH7664kTc9vT","page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a":"ref":19258984,"uniqueHTMLId":"page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a","name":"5e8391d0c088a","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.responsivecolumns","data":"columns":"2","className":"responsivecolumns","widget":,"uniqueId":"OojpQuJbrhBxFuS","parentUniqueId":"24M33ffWNaPiMQt","page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a-zones__5e8391d0c1462":"ref":19258985,"uniqueHTMLId":"page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a-zones__5e8391d0c1462","name":"5e8391d0c1462","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.columns.column","data":[],"className":"columns.column","widget":,"uniqueId":"SqnTWZ8sC6gR3CD","parentUniqueId":"OojpQuJbrhBxFuS","page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a-zones__5e8391d0c1462-widgets__5e8391d0c1c66":"ref":19258986,"uniqueHTMLId":"page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a-zones__5e8391d0c1462-widgets__5e8391d0c1c66","name":"5e8391d0c1c66","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.youtube","data":"videoId":"qB1xKkRcZ6Q","uniqueId":"mixit9kUZOfeNmy","userInput":"https:\/\/www.youtube.com\/watch?v=qB1xKkRcZ6Q","className":"youtube","widget":,"uniqueId":"mixit9kUZOfeNmy","parentUniqueId":"SqnTWZ8sC6gR3CD","page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a-zones__5e8391d0c27a9":"ref":19258987,"uniqueHTMLId":"page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a-zones__5e8391d0c27a9","name":"5e8391d0c27a9","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.columns.column","data":[],"className":"columns.column","widget":,"uniqueId":"7nsrJVr0gF24i5G","parentUniqueId":"OojpQuJbrhBxFuS","page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a-zones__5e8391d0c27a9-widgets__5e8391d0c2ef1":"ref":19258988,"uniqueHTMLId":"page-zones__main-widgets__5e8391d0b43f7-widgets__5e8391d0c088a-zones__5e8391d0c27a9-widgets__5e8391d0c2ef1","name":"5e8391d0c2ef1","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.contentgroup","data":"displayImage":"false","contentLayout":"regular","alignmentClass":"left","displayHeading":"true","headingType":"h2","headingContent":"Surgical Video: READ BEFORE CONTINUINGThe scenes reproduced in the surgical video on the page below may disturb the sensitivity of visitors.
WHO DECIDES TO ACCESS THE SURGICAL MOVIES IMPLICITLY AGREES TO THE VISION","displayParagraph":"true","bodyContent":"Based on the anatomical situation and neurological conditions of quadriplegic patients, Carlo SACCO M.D. performs various types of \"NERVE TRANSFER\" surgery in order to recover the use of the hands.
In the surgical video, a brachial branch of the MUSCULOCUTANEOUS NERVE is inserted for the ANTERIOR INTEROSSEOUS NERVE to be able to recover the nail flexion of the thumb and index finger.
BY CLICKING ON THE IMAGE NEXT TO THE TEXT YOU EXIT THIS WEB SITE AND ACCESS THE VANNI VERONESI YOUTUBE CHANNEL
This video is subject to age restrictions and is only available on YouTube.
English translation of the intraoperative comment in Italian:Patient of 35 years, six months ago spinal cord trauma with dive C4 fracture, spinal cord injury level C5.
We are doing nerve transfer surgery to try to recover the use of the fingers.
In the surgical field, the median nerve, the branch for the anterior interosseous nerve, the musculocutaneous nerve with the branch for the brachial muscle have been isolated.
The nerves exposed in the surgical field are stimulated at low intensity to demonstrate muscle movement in the brachial muscle and the long flexor muscle of the thumb.
The nerve transfer is then performed from a brachial branch of the musculocutaneous nerve pro anterior interosseous nerve.
THIS VIDEO IS MADE FOR EDUCATIONAL PURPOSES ONLY. PLEASE CONSULT YOUR DOCTOR BEFORE MAKING CARE DECISIONS.
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","displayLink":"false","uniqueId":"refYiQ3E49R28L2","description":"assets","linkUrl":"none","target":"none","action":"none","scale":"original","imageWidth":"auto","alt":"","title":"assets","align":"widget-align-left","paragraphType":"div","className":"contentgroup","widget":,"uniqueId":"refYiQ3E49R28L2","parentUniqueId":"VgZDAgtlvcVg0Fx","page-zones__main-widgets__5e83950762d47-widgets__5e8395077023b-zones__5e83950774516-widgets__5e83950778274":"ref":19258998,"uniqueHTMLId":"page-zones__main-widgets__5e83950762d47-widgets__5e8395077023b-zones__5e83950774516-widgets__5e83950778274","name":"5e83950778274","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.button","data":"action":"file","text":"CLICK HERE TO DOWNLOAD THE REST OF CARLINO ARTICLE IN ITALIAN - NEWS CITY OF LUGO (RA) - IN PDF FORMAT","uniqueId":"CG70RGR2pgc5vV3","target":"_self","url":"http:\/\/files.spazioweb.it\/6d\/6c\/6d6ccbed-3979-4dd4-bc2f-4f8c5746fdc3.pdf","align":"widget-align-left","fileName":"Dr. Sacco Resto del Carlino 2020.pdf","className":"button","widget":,"uniqueId":"CG70RGR2pgc5vV3","parentUniqueId":"VgZDAgtlvcVg0Fx","page-zones__main-widgets__5e839217d6a88":"ref":19258989,"uniqueHTMLId":"page-zones__main-widgets__5e839217d6a88","name":"5e839217d6a88","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.row","data":"backgroundType":"color","height":"default","horizontalAlignment":"center","layout":"","width":"contained","style":"dark","verticalAlignment":"top","localClass":"widget-row-70FU8B","uniqueId":"zQcNdQ09oHXZZpZ","className":"row","widget":,"uniqueId":"V1OUhQkaISKCooT","parentUniqueId":"3lEwH7664kTc9vT","page-zones__main-widgets__5e839217d6a88-widgets__5e839217e8e23":"ref":19258990,"uniqueHTMLId":"page-zones__main-widgets__5e839217d6a88-widgets__5e839217e8e23","name":"5e839217e8e23","fixed":false,"libraryItemRef":0,"pageRef":1330234,"temporary":,"changed":,"type":"widget.content","data":"type":"widget.content","content":"LAST UPDATE 6th MAY 2022","localClass":"widget-content-8TQY00","uniqueId":"Fe0nxjopQQfbT8a","className":"content","widget":,"uniqueId":"Fe0nxjopQQfbT8a","parentUniqueId":"V1OUhQkaISKCooT","page-zones__footer":"ref":19259062,"uniqueHTMLId":"page-zones__footer","name":"footer","fixed":false,"libraryItemRef":0,"pageRef":null,"temporary":,"changed":,"type":"widget.template","data":[],"className":"template","widget":,"uniqueId":"ub1NYKeBWUR0cMK","parentUniqueId":null,"page-zones__footer-widgets__5e88c2873c11e":"ref":19259063,"uniqueHTMLId":"page-zones__footer-widgets__5e88c2873c11e","name":"5e88c2873c11e","fixed":false,"libraryItemRef":0,"pageRef":null,"temporary":,"changed":,"type":"widget.row","data":"backgroundType":"color","height":"thin","horizontalAlignment":"center","width":"contained","style":"dark","verticalAlignment":"center","rowType":"footer-5","originalRowTemplateId":"footer_5","className":"row","widget":,"uniqueId":"F4KEDRrWfkMWYdX","parentUniqueId":"ub1NYKeBWUR0cMK","page-zones__footer-widgets__5e88c2873c11e-widgets__5e88c2873e8a6":"ref":19259064,"uniqueHTMLId":"page-zones__footer-widgets__5e88c2873c11e-widgets__5e88c2873e8a6","name":"5e88c2873e8a6","fixed":false,"libraryItemRef":0,"pageRef":null,"temporary":,"changed":,"type":"widget.contentgroup","data":"contentLayout":"regular","alignmentClass":"center","displayParagraph":"true","bodyContent":"\u00a9 Copyright 2020 Vanni VERONESI. All rights reserved.
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NERVE TRANSFER SURGERY","uniqueId":"QWw5hIGXTcgAoHM","type":"widget.content","fixed":false,"isInRow":true,"isNew":false,"isTemplateWidget":false,"name":"62751bad46f8b","pageRef":1330234,"parentUniqueId":"YBFwqtQ2WtZoQ0P","ref":33768786,"type":"widget.content","uniqueHTMLId":"page-zones__main-widgets__62751bad363b9-widgets__62751bad43926-zones__62751bad467d5-widgets__62751bad46f8b","uniqueId":"QWw5hIGXTcgAoHM","isZone":false,"parentZone":"main","zoneName":null,"className":"contentgroup","data":"displayImage":"false","contentLayout":"regular","alignmentClass":"left","displayHeading":"true","headingType":"h2","headingContent":"Quadriplegic patients recover the use of their hands","displayParagraph":"true","bodyContent":"In May 2012, the news of a quadriplegic patient who after a surgery in the USA at the Barnes-Jewish Hospital in St. Louis, after a year of rehabilitation, was able to bend the thumb and index finger of both hands https:\/\/www.corriere.it\/salute\/disabilita\/12_maggio_15\/paraplegico-muove-mani-usa_4ff6defe-9e91-11e1...In 2019 the news was released that in Turin, with a brand new surgical technique used in a few centres in the world, they have given back to a quadriplegic patient the use of their hands. They describe it as the first surgery of its kind performed in Italy. Widely disseminated the news on the foremost newscasts and websites such as TGcom24, ANSA, la Repubblica, social networks, etc. One news title: \"Turin, quadriplegic recovers the use of hands with an innovative intervention. The method, performed in a few centres in the world and for the first time in Italy, bypasses the lesion of the spinal cord by reconnecting healthy nerves like electrical wires.\"
In reality, the results can only be evaluated after 8-10 months as correctly reported in the news of the American patient,
In our team, my colleague Dr Sacco Carlo has carried out that type of surgery in quadriplegic patients to recover the use of their hands since 2014. However, in June 2019, the date of the surgery in Turin, Dr Sacco Carlo already had a case series of about ten patients; therefore, the first surgery in Italy, to be honest, was performed in Romagna in 2014.
In the following months and years, our operated patients underwent a clinical and rehabilitation check in collaboration with colleagues from the Montecatone Institute.
We have published these surgical and rehabilitative treatments in the scientific journal \u201cMicrosurgery\u201d. The series consists of a group of 11 patients with a treatment of 14 upper limbs and a postoperative observation period of at least 12 months.
One year after surgery, the results show that nerve transfer surgical procedures have significantly improved muscle strength and functional outcomes. Therefore we believe that surgical nerve transfer can represent a milestone in the rehabilitation process.
To appreciate the results of the surgical treatment and the subsequent rehabilitation treatment with the recovery of hand movements, you can view the video, kindly granted by Dr. Olivi Silvia, Head of the Specialist Hospitalization Unit of the MRI of the Montecatone Institute, by clicking in the box 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patients who can benefit from the particular surgical technique of NERVE TRANSFER to the upper limbs to recover the use of the hands. In addition to selection based on clinical conditions, the most appropriate period for surgical treatment should also be evaluated. The results of the surgery can be seen after about a year, while waiting the patient will be followed and subjected to adequate physiotherapy.
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WHO DECIDES TO ACCESS THE SURGICAL MOVIES IMPLICITLY AGREES TO THE VISION","displayParagraph":"true","bodyContent":"Based on the anatomical situation and neurological conditions of quadriplegic patients, Carlo SACCO M.D. performs various types of \"NERVE TRANSFER\" surgery in order to recover the use of the hands.
In the surgical video, a brachial branch of the MUSCULOCUTANEOUS NERVE is inserted for the ANTERIOR INTEROSSEOUS NERVE to be able to recover the nail flexion of the thumb and index finger.
BY CLICKING ON THE IMAGE NEXT TO THE TEXT YOU EXIT THIS WEB SITE AND ACCESS THE VANNI VERONESI YOUTUBE CHANNEL
This video is subject to age restrictions and is only available on YouTube.
English translation of the intraoperative comment in Italian:Patient of 35 years, six months ago spinal cord trauma with dive C4 fracture, spinal cord injury level C5.
We are doing nerve transfer surgery to try to recover the use of the fingers.
In the surgical field, the median nerve, the branch for the anterior interosseous nerve, the musculocutaneous nerve with the branch for the brachial muscle have been isolated.
The nerves exposed in the surgical field are stimulated at low intensity to demonstrate muscle movement in the brachial muscle and the long flexor muscle of the thumb.
The nerve transfer is then performed from a brachial branch of the musculocutaneous nerve pro anterior interosseous nerve.
THIS VIDEO IS MADE FOR EDUCATIONAL PURPOSES ONLY. PLEASE CONSULT YOUR DOCTOR BEFORE MAKING CARE DECISIONS.
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