Generating comBOs under normoxic conditions

[Alyssa Monique Monterroso]

Hi All,

I hope this email finds everyone well!

Just reaching out with a quick question. Has anyone tried to generate comBOs under normoxic conditions and done a comparison to comBOs generated under hypoxic conditions? If yes, how did they compare?

Thank you all for reading over my question and wishing everyone a great day! 🙂

Kind Regards, 

Alyssa 

[Abdullah Khan]

Hi Alyssa, 

Thanks for your emai!

We have and the major differences is: 

- You don't get an efficient induction of osteolineage cells in particular

- Reduce vascularisation (hypoxia favours this) 

- Lymphoid induction also favoured by the low oxygen conditions (+ IL7) 

- HSPC/HSC behaviours quite different (type better with adult post low O2 as per pre-print, we've fleshed this out more in the revisions we are working on so watch this space). 

Note that if you're interested it's worth looking at the original BMO paper (Khan et a. Cancer Discovery) and the Frenz-Weissner BMP paper (Nature Methods). Frenz Weissner don't use low O2, and their data/single cell at day 45 and flow are quite different.

Thanks for TooTing! 

Abs