Primary cell engraftment using adjusted hydrogel method

[Karolin Stumpf]

Hi all,

did anyone compare the original hydrogel method (12-well plate from D15-D12 of diff protocol) to the new microgranular gel method (directly embedding aggregates on D5 in a 96 well plate) in terms of facilitation of primary cell engraftment?
I am witnessing slightly lower engraftment percentages/numbers after the same number of days of co-culture with the microgranular gel.

Would be very thankful if anyone could comment on their experiences:)

Best

Karo