I am new to scRNAseq analysis and just found out about monocle.
I have a large dataset from a paper, raw data (~340 fastq files) and processed data with rpm values.
I can do alignment and cufflinks on those fastq files.. but it will take forever to just fastqdump those files
can I use rpm values for monocle? has anyone tried using monocle with rpm values?
any help would be much appreciated!
thanks