How do I change the tautomeric form of Histidine in a structure?

[Andrew Orry]

Q.

How do I change the tautomeric form of Histidine in a structure?

A.

• Read in and display the PDB structure
• Convert to ICM Object.
• Right click on the residue you wish to mutate. If you wish to mutate the C or N terminal please see the next question.
• Select Advanced/Mutate Amino Acid
• Select one of the following: Hip(histidine protonated), Hie (histidine epsilon tautomer)