How to mutate multiple residues to Ala.

[Andrew Orry]

Q.

How to mutate multiple residues to Ala.

A.

You can do this on the command line or in GUI. In both cases your PDB structure needs to be an ICM object.

In the example below we convert all histidine residues in different forms (his,hie and hip) to alanine in the pdb 1xbb.

In the command line:

# read and convert
read pdb "1xbb"
convertObject a_ no yes yes no

# mutate histidines to alanine

mutateResidue a_/his,hie,hip "ala"

In the GUI:

- Read in a pdb structure e.g. 1xbb

- Convert to ICM object

- Select the residues you want to mutate

- Right click on a selection and choose Advanced->mutate residue(s) and enter the 3-letter amino acid code