Using mod_wsgi-express start-server was never supported on Windows as I could never get it to work properly as I lacked sufficient knowledge of the Windows platform to get it working. When I say “supported” that means I cannot help you with it. The code sort of half worked, but rather than totally disable it I left it as is with the hope that someone may help to get it fully working, but no one has ever stepped up to do that.
I would be surprised that mod_wsgi as a whole works on Windows any more even when integrated manually into Apache httpd. I personally haven’t built or tested it on Windows in well over 5 years. I would expect that changes in how Python is installed on Windows, as well as how Python virtual environments are constructed on Windows would have broken things by now, as these sorts of things kept changing and fiddles were needed to keep it running. I suspect that this is what you are seeing. Windows has likely changed how virtual environments are setup once again and the Python library DLL (.pyd), is no longer located in the same spot.
So there isn’t really anything I can do to help you directly. If you really wanted to get it working I can only suggest digging through the mod_wsgi-express startup script and see if you can work out what needs to change for it to support the latest Python versions and virtual environment layout.