[micro-manager-general] Question about Retiga 6000 and Retiga 6

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Cammer, Michael via micro-manager-general

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Mar 12, 2020, 6:09:42 PM3/12/20
to micro-mana...@lists.sourceforge.net, Cammer, Michael

I have set up two different MicroManager installations for people with a Retiga 6 on one and a Retiga 6000 on another on Windows 10 computers.


On both systems, 1 x 1 binning of full field does not work.  I am guessing that this is due to the large number of pixels and limitations of the USB bus speed.  I was wondering whether there is a way to set MM to delay between each frame in live mode to allow for full frame 1 x 1 imaging or if there is another way to make this work. 


If not, help would be appreciated with presetting a ROI for imaging field.


Thank you!


Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016

Michael...@med.nyu.edu  http://nyulmc.org/micros  http://microscopynotes.com/

Voice direct only, no text or messages:  1-914-309-3270 and 1-646-501-0567

 

Cammer, Michael via micro-manager-general

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Mar 12, 2020, 7:04:51 PM3/12/20
to Micro-Manager General, Cammer, Michael

Thank you.


Teledyne/Qimaging was very helpful with setting up the Retiga 6000 with a version of pvcam specifically for Win10.  Except for this speed issue.


I reached out to you to see if there was any MM issues specifically.  Teledyne/Qimaging gave me some avenues to investigate to troubleshoot.  And I contacted the IT people to see if they installed anything that may be causing a bottleneck, like anti-virus.


What I was thinking for ROI was a preset so that we don;t have to take a snapshot and then draw a ROI.  Like a preset 1200 X 1200 at the center when starting up a configuration.


Thank you again and best regards-


Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016

Michael...@med.nyu.edu  http://nyulmc.org/micros  http://microscopynotes.com/

Voice direct only, no text or messages:  1-914-309-3270 and 1-646-501-0567

 

From: Nico Stuurman <nico.s...@ucsf.edu>
Sent: Thursday, March 12, 2020 6:33:08 PM
To: Micro-Manager General
Subject: Re: [micro-manager-general] Question about Retiga 6000 and Retiga 6
 
[EXTERNAL]

Hi Michael,


> I have set up two different MicroManager installations for people with
> a Retiga 6 on one and a Retiga 6000 on another on Windows 10 computers.
>

Those are Teledyne/Qimaging cameras working through PVCAM?

>
>
> On both systems, 1 x 1 binning of full field does not work. I am
> guessing that this is due to the large number of pixels and
> limitations of the USB bus speed.  I was wondering whether there is a
> way to set MM to delay between each frame in live mode to allow for
> full frame 1 x 1 imaging or if there is another way to make this work.
>

It may be useful to ask Teledyne for support.  They have been very
supportive of Micro-Manager and may be able to give good advice and/or
fix problems when asked.


> If not, help would be appreciated with presetting a ROI for imaging field.
>

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