You may not want to use the sdcard for your pictures as each user of the app will not be able to access the pictures unless your app has a way to save them on their device.
I would put the pictures in the Media folder instead.
However, to answer your question,
If you placed Image01.jpg directly in the sdcard folder you access it this way: "/Image01.jpg"
If you keep all of your images in a subfolder under 'sdcard' named 'images' then you would use: "/images/Image01.jpg"
If you place it in the app's Media folder you would use: "Image01.jpg"
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sf